NY/T 3060.2-2016 in English
VALIDCode of practice for evaluation of barley varieties for resistance to disease-Part 2:Powdery mildew
- Issued on:2016-12-23
- Implemented on:2017-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
Price(USD)：
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| Standard No: | NY/T 3060.2-2016 |
| Document status: | VALID |
| Title in English: | Code of practice for evaluation of barley varieties for resistance to disease-Part 2:Powdery mildew |
| Title in Chinese: | 大麦品种抗病性鉴定技术规程 第2部分：抗白粉病 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2016-12-23 |
| Implemented on: | 2017-04-01 |
| Professional Classification: | NY-Agriculture |
| Related Keywords: | lesions high resistance
resistance level resistance evaluation standards infection type lesion characteristics barley varieties resistance genes |
| Related Topics: | broad spectrum disease resistance
broad spectrum disease resistance disease resistance powdery mildew barley variety disease resistance automatic disease resistance common disease which flour is white ny/t2997-2016 Comparison of identification methods of wheat powdery mildew |
Introduction
Analysis of standard technical framework
| Core elements | Identification at the seedling stage | Identification at the mature plant stage |
|---|---|---|
| Identification site | Plastic square box/pot/petri dish | Field disease nursery |
| Key indicators | Infection type (0-4 levels) | Severity (0-9 levels) |
| Inoculation requirements | Mix and sweep method during the single leaf expansion period | Transplanting of diseased seedlings in the early stage of jointing |
Key technical points of pathogens
Dual paths for strain isolation:
- Asexual generation: Through three cycles of single spore pile purification, preserved in benzimidazole medium (50-60μg/mL)
- Sexual generation: Ascospore release was induced by immersion in closed thecocysts, and 16h light/8h dark alternating culture was required
Typical application case: Yunnan Baoshan Experimental Station adopted the mixed strain inoculation method, inoculated three dominant virulence strains (Bgh-YN1/Bgh-YN3/Bgh-YN5) in a 1:1:1 ratio, and accurately identified the resistant variety Yunnan Barley No. 12.
Detailed explanation of resistance evaluation standards
| Infection type | Lesion characteristics | Corresponding resistance level |
|---|---|---|
| 0 | No visible lesions | Immune (IM) |
| 1 | Lesions <1mm, mycelium transparent green | High resistance (HR) |
| 3 | Lesions >1mm, mycelium layer thick | Moderately susceptible (MS) |
Suggestions for the implementation of the standard
- Strain update:Field standard samples should be collected again every 2 years for toxicity spectrum verification
- Control setting:Must include varieties with known resistance genes (such as Mla3/Mla6 carrier varieties)
- Data recording:It is recommended to use Appendix B form + digital image dual recording
Technology evolution analysis
Compared with the 2008 version of the standard, the 2016 version has the following major improvements:
- Added Closed capsule separation technology process
- Refine the 9-level severity classification standard
- Clear the requirements for mixed strain inoculation
Sample only — not a preview of NY/T 3060.2-2016
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