SN/T 1204-2016 in English

VALID

Protocol of the real-time PCR method for detecting genetically modified plants and their derived products

Issued on:2016-06-28
Implemented on:2017-02-01
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $490.00
$476.00

Standard No:	SN/T 1204-2016
Document status:	VALID
Title in English:	Protocol of the real-time PCR method for detecting genetically modified plants and their derived products
Title in Chinese:	植物及其加工产品中转基因成分实时荧光PCR定性检验方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2016-06-28
Implemented on:	2017-02-01
Superseding:	SN/T 1204-2003 Protocol of the real-time PCR for detecting genetically modified plants and their derived products
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	real-time fluorescence pcr instrument real-time pcr method positive control ct value ≤35 negative control ≥40 standard detection objectsnew content
Related Topics:	fluorescent pcr genetically modified ingredients genetically modified pcr amplification fluorescent pcr sn/t1204 Timed Fluorometric Quantitative Method Multiplex fluorescent PCR transgenic components Fluorescent real-time PCR Fluorescent quantitative PCR analysis method real-time fluorescence pcr sample Real-time fluorescent PCR PCR qualitative Guidelines for the development of qualitative PCR methods for the detection of components of transgenic plants and their products Guidelines for the development of real-time fluorescent quantitative PCR methods for the detection of components in transgenic plants and their products pcr transient strain specificity Qualitative PCR method for the detection of reporter genes gus and gfp in transgenic plants and their products Real-time fluorescence quantitative PCR analysis strain specificity u6 gene in plants Real-time fluorescent PCR PCR qualitative genetically modified ingredients pcr amplification Processed products Processed products Fluorescent quantitative PCR analysis method Analytical method for real-time fluorescence quantification PCR gene amplification and detection Fluorescent quantitative PCR in inspection and quarantine amplified pcr genetic testing methods pcr time gene Qualitative test Qualitative test suppressor gene transgenic plant Fluorescent quantitative PCR analysis method genetic component real-time fluorescence Fluorescent quantitative PCR analysis method genetic component Real-time fluorescent quantitative PCR analysis real-time pcr analysis method Fluorescent real-time PCR Testing of Plant Origin Products Fluorescence PCR instrument usage method Inspection of transgenic plants and their products transgenic plant Plant Genetics Laboratory Plant GMO Detection Transgenic pcr test method gene suppression Composition Standard gene suppression fluorescent pcr Methods for detection and identification of transgenic plants transgenic fluorescent plants "Genetically Modified pcr components Plant PCR instrument Transgene fluorescence quantification plant genetic approach fluorescence real-time plant genome dna extraction Element Aquatic bacteria fluorescent PCR Gene transcription level determination, analysis method after fluorescence real-time quantitative PCR PCR product entrainment Transformation effects and methods in experiments Methods for estimating the time of horizontal gene transfer What are the methods for detecting genetically modified plants? Testing methods for genetically modified foods Genotyping methods and principles Plant gene cloning methods What are the detection and identification methods for genetically modified plants? Chemokine Basic Experimental Methods Selection of plant transgenic methods Methods and principles of gene mapping sn/t1204-2004 Experimental methods for detecting gene expression include Plant transgenic cloning method

Introduction

Analysis of the core content of the standard

Detection objects	New content (2016 version)	Number of detected genes	Minimum detection limit
18 types of crops such as soybeans and corn	Endogenous genes + strain-specific detection	35+exogenous genes	0.01%
Processed products	DNA extraction optimization	7 groups of control systems	20ng/μL

Technology evolution analysis

Compared with the 2003 version, the 2016 version of the standard has the following major breakthroughs:

Expansion of detection scope: 8 new detection methods for crops such as flax and sugar beet
Technology upgrade: UNG enzyme anti-pollution system is adopted, and ROX fluorescence correction is added

System improvement: Standardized primer and probe sequences (Appendix A contains 39 sets of primer and probe combinations)

Key implementation points

Instrument selection: A real-time fluorescence PCR instrument must be equipped, and it is recommended to use a device with a ROX correction channel

Quality control requirements: Positive control Ct value ≤35, negative control ≥40, blank control no amplification

Determination threshold: Ct value ≤36 is considered positive, 36-40 requires retesting, ≥40 is considered negative

Application Cases

Take soybean Roundup Ready detection as an example:

After DNA extraction, Lectin endogenous gene and CP4-EPSPS exogenous gene

Verify using MON89788 strain-specific primers in Appendix B

When the Ct value is 28 (exogenous) and 25 (endogenous), it is determined to be GMO positive

More samples: GB 50021-2001(2009) JC/T 2453-2018 DL/T 5154-2012

Sample only — not a preview of SN/T 1204-2016

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