SN/T 4671-2016 in English

VALID

Determination of glyphosate and its metabolite residue in cotton for import and export―Liquid chromatography-tandem mass spectrometry method

Issued on:2016-12-12
Implemented on:2017-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	SN/T 4671-2016
Document status:	VALID
Title in English:	Determination of glyphosate and its metabolite residue in cotton for import and export―Liquid chromatography-tandem mass spectrometry method
Title in Chinese:	进出口棉花中草甘膦及其代谢物残留量的测定液相色谱一质谱/质谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2016-12-12
Implemented on:	2017-07-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	liquid chromatography-tandem mass spectrometry chromatography-tandem mass spectrometry method introduction technical background mass spectrometry detection minutes mass spectrometer parameters glyphosate recoveries
Related Topics:	How to Analyze Mass Spectrometry by Liquid Chromatography No peak in liquid chromatography Liquid chromatography does not show Chromatographic coelution Imported gas chromatograph No peak in mass spectrum Pharmacokinetic mass spectrometry Imported liquid phase The auto-spectrum and cross-spectrum of Imported liquid phase Imported liquid chromatography Phosphonium salt mass spectrometry Essence Spectrum mass spectrometry hplc Essence Spectrum MS residue Liquid Chromatography Mass Spectrometry Glyphosate No peak in mass spectrum Oxalic acid mass spectrometry mass spectrometry semi-quantitative semi-quantitative mass spectrometry No peak in liquid chromatography Mass spectrometric detection of metabolites mass spectrometry metabolism quantitative mass spectrometry Quantity measured by mass spectrometry Liquid phase and spectroscopy no chromatographic peak Chromatographic peak Chromatographic peak mass spectrometry semi-quantitative semi-quantitative mass spectrometry cotton spectrum cotton Mass Spectrometry No peak in gas chromatography mass spectrometry GC mass spectrometry does not produce peaks from time to time membrane mass spectrometry Spectrum material Mass Spec Metabolites neutral mass spectrum MS mass 18 Ground Object Spectrum and Its Measurement mass spectrometry Chromatographic round peak MS co-elution LC-MS co-elution Liquid chromatography + peak extraction Liquid chromatography peaks within a few minutes at the earliest Liquid chromatography peaks in 70 minutes mass spectrum peak MS effluent Mass Spectrometry of Metabolites Spectrum and its spectrum Imported chromatography Normal phase chromatography + peak extraction Liquid chromatography peaks in one minute Quantification by mass spectrometry LC peaks prematurely MS co-elution Improvements in mass spectrometry Other mass spectrometry techniques Terms in Mass Spectrometry Mass Spectrometry Liquid inlet Liquid Chromatography Peak MS residue Absolute quantitation by mass spectrometry LC outlet ring Mass Spectrometry Internal Quantification No peak in normal phase chromatography Mass Spectrometry + Determination + Quantity liquid measurable substance Absolute quantitation by mass spectrometry Glyphosate liquid chromatography mass spectrometry Mass spectrometry + semi-quantitative A chromatographic peak magnetic mass spectrometry mass spec metabolites Quantitative mass spectrometry Mass Spectrum 41 Peak Chromatographic mass fraction Mass Spectrometry Metabolic Detection MS mass 36 Quantitative Phosphine Spectroscopy Imported gas chromatograph The liquid chromatography had a peak last time and no peak this time Oxalic acid mass spectrometry Excellent Energy Metabolism Metabolite Chromatographic Peaks Metabolic primary mass spectrometry secondary High Quality for Energy Metabolism Assays Mass Spectrometry flow cytometry mass spectrometry mass spectrometry metabolism Phosphine liquid mass method Mass Spectrometry and Mass Spectrometry Imaging Modern Mass Spectrometry Mass Analyzers Negative peaks appear in the chromatogram Mass spectrometry injection load Mobile phase for mass spectrometry The mass spectrometer cannot enter water Traditional Chinese medicine mass spectrometry Compounds with no peaks in the mass spectrum Standard poor quality metabolome Qualitative methods commonly used in chromatographic analysis and their characteristics Mass spectrometry parameters in quality standards Injection method for mass spectrometric titration

Introduction

Technical Background of the Standard

This standard, issued on December 12, 2016, and implemented on July 1, 2017, specifies the detection of glyphosate and its metabolites in cotton products using liquid chromatography-tandem mass spectrometry. The technique is based on the FMOCl derivatization reaction and quantitative analysis using an isotope internal standard method, with a detection limit of 0.05 mg/kg.

Core Detection Process

Steps	Key Parameters	Technical Points
Sample Pretreatment	10 g sample was crushed to 2 mm	Derivatization was performed using 1.0 μg/mL isotope internal standard solution
Derivatization Reaction	30 min oscillation reaction	Add 100 μL borate buffer and 50 μL FMOCl reagent
Chromatographic Conditions	C18 column (100 mm × 2.1 mm)	Gradient elution: 5 mM ammonium acetate/methanol

Method Validation Data

Standard validation showed that at spike levels of 0.05-0.20 mg/kg, glyphosate recoveries ranged from 75.2% to 96.4%, and metabolite recoveries ranged from 68.8% to 94.0%. Mass spectrometry detection was performed using a TSQ Quantum ULTRA AM system in positive ESI mode with collision energy optimized to 10-25 V.

Key Points

Strictly control the storage conditions of the derivatization reagent FMOCl (5°C, protected from light)
Note the retention time differences of the internal standard (¹³C₁₅N-labeled compound): glyphosate 9.8 minutes, metabolite 12.7 minutes
Mass spectrometer parameters need to be calibrated regularly to ensure that the response value deviation is ≤2.5%

More samples: JGJ 94-2008 DL/T 5154-2012 NB/T 47013.2-2015

Sample only — not a preview of SN/T 4671-2016

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

SN 0283-1994 in English

\"Method for Inspection of Dichlorodipyridinol Residues in Exported Poultry Meat\"
1994-02-18
SN/T 3547-2013 in English

Food contact materials - Polymers determination of styrene monomer - Gas chromatography
2013-03-01
SN/T 0247.1-2015 in English

Rules for the inspection of electrical machines for export - Part 1: General requirements
2015-09-02
SN 184-1993 in English

\"Test Methods for Listeria Monocytogenes in Exported Foods\"
1993-10-08
SN/T 2297.1-2009 in English

Chemical analysis methods of gypsum and gypsum products for import and export—Part 1:Determination of strontium content
2010-07-07
SN/T 1446-2010 in English

Quarantine protocol for transmissible gastroenteritis of swine
2010-05-27
SN/T 3334.3-2013 in English

Determination of bisphenol A in the household appliances - LC-MS/MS
2013-03-01
SN/T 1751.3-2011 in English

Determination of quinolone residues in food of animal origin for import and export—Part 3:HPLC method
2011-02-25
SN/T 0894-2000 in English

Rules for the inspection of safety requirements of household and similar appliances for hair care for export
2000-06-22
SN/T 0244-1994 in English

\"General Rules for Inspection and Certification of Imported Mechanical and Electrical Instruments\"
1993-08-01