SN/T 4675.8-2016 in English
VALIDDetermination of 5-hydroxymethylfurfural in wine for export-HPLC method
- Issued on:2016-12-12
- Implemented on:2017-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$194.00
Introduction
Standard Overview and Technical Background
SN/T 4675.8-2016, a key component of the import and export wine inspection standards, specifically specifies the method for determining 5-hydroxymethylfurfural (5-HMF) using a high-performance liquid chromatograph (HPLC). 5-HMF is a Maillard reaction product, and its content can reflect the wine's heat treatment and storage conditions.
Principle and core technology of the method
| Technical parameters | SN/T 4675.8 requirements | Comparison with international OIV standards |
|---|---|---|
| Detection wavelength | 284nm | 285nm |
| Chromatographic column | C18(250mm×4.6mm,5μm) | C18(150mm×4.6mm,5μm) |
| Mobile phase | Methanol+water(1+9) | Acetonitrile + Water (5+95) |
Key Operation Procedures
Sample Pretreatment: Take 10.0 mL of alcohol sample and dilute to 100 mL with 10% methanol solution. Filter through a 0.45 μm filter before injection. The standard curve should cover a concentration range of 0.1-5.0 mg/L.
Chromatographic Condition Optimization: Under the conditions of a column temperature of 30°C, a flow rate of 1.0 mL/min, and an injection volume of 10 μL, the retention time of 5-HMF should be stable at 10.6 ± 0.3 minutes.
Method Validation Data
Standard validation showed that: within the concentration range of 1.0-20.0 mg/L, the spiked recoveries in different matrices were 92.8%-109%, with relative standard deviations (RSDs) less than 5%. The method detection limit was 1.0 mg/L, and the limit of quantification was 2.1 mg/L.
Implementation Recommendations
- Regularly verify the stability of the chromatographic system using reference materials.
- Samples should be stored away from light and tested within 24 hours.
- Blank tests and quality control samples should be performed simultaneously with each batch of samples.
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