SN/T 4726-2016 in English

Standard Background and Technological Evolution

SN/T 4726-2016 is the quarantine and identification industry standard formulated by my country for Adoxophyes orana (apple tormenter). As an important cross-border agricultural pest, the identification technology of this species has undergone three major iterations:

Analysis of core identification characteristics

Morphological identification of adults

According to clause 7.2.1 of the standard, adults of Adoxophyes orana have the following key characteristics:

• Forewings are 13-23mm, with a yellowish-brown to tan base
• Wings have typical reticulated patterns and three silvery horizontal bands
• Hindwings are off-white with distinct cilia

Technical specifications for molecular detection

Appendix E of the standard specifies the molecular identification process:

1. DNA extraction: chest muscle tissue is processed using the CTAB method
2. PCR amplification: primer sequence COI-F:5'-GGTCAACAAATCATAAAGAT-3'
3. Electrophoresis analysis: 1.5% agarose gel, expected band 658bp

Implementation recommendations

Laboratory configuration requirements

Common misjudgment avoidance

Pay special attention to distinguish it from Adoxophyes honmai:

• The terminal process of the male genitalia of the latter is hook-shaped
• The silvery horizontal band on the forewing is not clearly interrupted