SN/T 4793-2017 in English

VALID

Test methods for Plasmodium by real time PCR at ports

Issued on:2017-05-12
Implemented on:2017-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $120.00
$117.00

Standard No:	SN/T 4793-2017
Document status:	VALID
Title in English:	Test methods for Plasmodium by real time PCR at ports
Title in Chinese:	国境口岸疟原虫实时荧光PCR检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-05-12
Implemented on:	2017-12-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	plasmodium species human plasmodium real time pcr plasmodium falciparum specific fragment fam-bhq1 detection time
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Introduction

Analysis of the core content of the standard

This standard establishes a complete molecular detection system for Plasmodium, using equipment such as fluorescence quantitative PCR instrument to achieve differential diagnosis of four types of human Plasmodium through specific primer and probe combinations. Technical points include:

Assay Type	Target Gene	Probe Type	Melting Temperature (℃)
Universal Assay	Conserved Sequence	FAM-MGB	-
Plasmodium falciparum	Specific Fragment	FAM-BHQ1	-
Plasmodium ovale	Species Specific Sequence	SYBR Green	72.5

Key Technical Parameters

Sample Requirements: 3-5 mL of venous blood should be collected into EDTA anticoagulant tubes and stored at -20°C to avoid repeated freezing and thawing.

Amplification System: 25 μL standard reaction volume, containing 12.5 μL 2× MasterMix and specific primers and probes.

Cycling Parameters: Initial denaturation at 95°C for 5 min, followed by 40 cycles (95°C for 10 s → 58°C for 45 s).

Implementation Points

1. Biosafety: Experimental operations must be performed in a Class IIA Biosafety Cabinet in accordance with GB19489 requirements.

2. Quality Control Requirements: Negative and positive controls must be set up, and the Ct value of the positive control should be <35

3. Result Interpretation:

Positive: Ct ≤ 35 and a typical amplification curve
Suspicious: 35
Negative: No amplification curve

Application Value of the Standard

This standard establishes a standardized molecular detection process for malaria at ports of entry for the first time. Compared with traditional microscopy methods:

Sensitivity is increased by 100 times (0.1 parasites/μL blood can be detected)
Detection time is shortened to 4 hours
Accurate typing of the four Plasmodium species is achieved

More samples: QC/T 942-2021 JGJ 94-2008 DL/T 5154-2012

Sample only — not a preview of SN/T 4793-2017

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