SN/T 5114-2019 in English
VALIDDetermination of chloramphenicol in live animals and feed for import and export - LC-MS/MS
- Issued on:2019-09-03
- Implemented on:2020-03-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$127.00
Introduction
Analysis of the core content of the standard
| Technical elements | Animal tissue | Feed sample | Other matrices |
|---|---|---|---|
| Pretreatment differences | Acetonitrile extraction + centrifugation | Alkaline solution extraction + concentration | Buffer extraction + SPE cleanup |
| Limit of quantification (LOQ) | 0.05mg/kg | 0.1mg/kg | 0.5mg/kg |
| Recovery range | 85.8%-106.1% | 84.7%-99.5% | 81.7%-96.3% |
Technical points of the method
Chromatographic conditions:A Thermo Hypersil GOLD column (1.9μm, 2.1×100mm) was used, and the mobile phase was 5% formic acid aqueous solution (A)-acetonitrile (B) gradient elution at a flow rate of 0.3mL/min.
Mass spectrometry parameters:ESI positive ion mode, monitoring ion pairs 356.05/185.02 (quantitative ion) and 356.05/335.9 (qualitative ion), and the collision energies were set to 20.5V and 6V, respectively.
Implementation and application recommendations
- Different matrix samples must strictly select the corresponding pre-treatment method according to the standard. Animal tissue samples are recommended to be treated with a high-speed refrigerated centrifuge
- When tuning the mass spectrometer, ensure that the response intensity of the parent ion 356.05 is ≥95%, and the secondary mass spectrometry fragment ratio should meet the requirements of Appendix C
- The standard solution must be stored at -15℃～-20℃ away from light, and the working solution must be prepared and used immediately
Technology evolution analysis
This standard has the following major improvements compared to GB/T 20195-2006: ① The detection limit is reduced by 10 times; ② A new feed matrix treatment method is added; ③ The UPLC-MS/MS technology is used to increase throughput. Since its implementation in 2020, it has become the main basis for the supervision of florfenicol in imported and exported animal products.
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