SN/T 5275-2019 in English
VALIDProtocol of technique identification for ivory and worked ivory
- Issued on:2019-12-27
- Implemented on:2020-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$204.00
| Standard No: | SN/T 5275-2019 |
| Document status: | VALID |
| Title in English: | Protocol of technique identification for ivory and worked ivory |
| Title in Chinese: | 象牙及其制品鉴定技术规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2019-12-27 |
| Implemented on: | 2020-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Topics: | elephant
ivory red ivory object identification Noodles and their products Document Production Time Identification Technical Specification Identification sn/t 2019 tooth |
Introduction
Analysis of standard technical framework
| Technical dimensions | Morphological identification | Molecular biology identification |
|---|---|---|
| Core indicators | Schwartz structure, outer edge Schwartz angle, fluorescence reaction | Cytb gene sequence alignment |
| Detection accuracy | Species differentiation accuracy 85-90% | Species differentiation accuracy>98.5% |
| Applicable scenarios | Preliminary screening and identification of complete samples | Confirmation of trace/degraded samples |
Detailed explanation of key identification technologies
1. Steiner structure characteristic system
The standard innovatively establishes a three-dimensional Steiner structure evaluation system:
- Cross-sectional characteristics:Network/pointed-angle texture radiating from the center
- Longitudinal section characteristics:Intermittent linear or wavy texture
- Quantitative indicators:The measurement of the outer edge Steiner angle requires the use of Image J software for accurate determination
2. Key points of molecular detection technology
Nested PCR detection adopts a two-round amplification strategy:
- The first round amplifies a 472bp fragment (primer Cytb F/R1)
- Second round amplification of 450bp fragment (primer Cytb F/R2)
Typical positive judgment requires the following: electrophoresis band size matches and sequence homology> is 98.5%
Recommendations for standard implementation
1. Laboratory configuration requirements
Recommended equipment:
- stereo microscope (40-100 times magnification)
- UV lamp (365nm wavelength)
- PCR instrument and electrophoresis system
2. Operational risk control
Precautions for ivory sample handling:
- Wear a protective mask when cutting
- Physically isolate the DNA extraction area from the PCR area
- Set up extraction negative control
Technology evolution analysis
Main progress of this standard compared with LY/T 2200:
- Add identification index of mammoth ivory
- Introduce quantitative measurement method of outer edge Sterling angle
- Optimize DNA extraction process (CTAB method)
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