SN/T 5366.1-2022 in English

VALID

Commercial kit method-Enterobacteriaceae count - Test method I

Issued on:2022-03-14
Implemented on:2022-10-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $190.00
$185.00

Standard No:	SN/T 5366.1-2022
Document status:	VALID
Title in English:	Commercial kit method-Enterobacteriaceae count - Test method I
Title in Chinese:	商品化试剂盒检测方法肠杆菌科计数方法一
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2022-03-14
Implemented on:	2022-10-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	commercial kit detection method test method traditional culture method standardized method reference method
Related Topics:	bacillus Reagent test kit Enterobacter Enterobacteriaceae enumeration Kidney function test kit Frontiers of Commodity Inspection Methods counting method Enterobacteriaceae Kit detection sn/t2022 test method Enterobacteriaceae Test Sheet commercialization Bacmid Kit Enterobacter test strip Liquid agent detection method Sample box design bacmid dna kit egfr detection kit Method Kit Escherichia coli number detection method box technology counting method test product 2 test product 2 test product 2 test product 2 test product 2 multiparameter kit Standard Box Reagent Escherichia coli number detection method Reagent detection method Non-statutory tested goods Determination of Escherichia coli detection method Enterobacteriaceae detection method Forensic Test Kit Unified detection method Mesh detection method Enterococcus detection method western notation Lithium Detection Kit Lithium Detection Kit Enterococcus detection method Enterococcus detection method box counting Is Enterobacteriaceae present? Color detection method Plasticizer detection method ATP kit usage Ki67 detection kit How to use the influenza test kit Detection method of brucellosis detection kit Enterobacteriaceae detection national standard number Limulus reagent quantitative detection method atp detection kit Escherichia coli fluorescence PCR detection kit Aquatic products special testing kit E. coli national standard detection method E. coli transformation method Nine-tube method for testing E. coli Principle and method of urine testing kit Test kit testing standards disclosed Cadmium kit detection method principle E. coli transformation method Flow cytometry reagent detection method Methods for transforming E. coli Testing kit Fungal enumeration method Plasticizer national standard detection method Methods for determining the quality of bacterial agents Optimize detection methods The kit is a non-standard method Copper reagent detection method What are the methods for detecting plasticizers? Color protectant detection method E. coli plasmid calcium chloride transformation method Tablet testing methods Detection methods for plasticizers Dissolution testing methods for enteric-coated preparations sn/t 5380-2022 Biochemical milliliter test number method Test kit industry E. coli test paper detection method Calculation Method for E. coli Detection in Food

Introduction

Analysis of the core content of the standard

This standard specifies the standardized method for enumerating Enterobacteriaceae in food using the 3M Petrifilm Enterobacteriaceae Count Plate. The technical points include:

Technical elements	Standard requirements	Implementation points
Sample processing	1:10 homogenate preparation	Homogenization speed8,000-10,000 rpm
Culture conditions	36±1℃ 24±2h	Test plate stacking ≤20 layers
Typical colony characteristics	Red colonies with yellow halo/bubbles (3 forms)

Key technical indicators

Linear validation data show that the correlation coefficient between the test piece and the reference method (GB 4789.41) is 0.986-0.999, including:

Meat product detection slope 1.0867 (beef filling)
Dairy product detection slope 0.9398 (cheese slices)

Implementation Precautions

pH adjustment range 6.5-7.5 (using 1mol/L NaOH/HCl)
After inoculation, stand for 1 minute to allow the liquid to diffuse
Special judgment is required when the background changes color or the gel liquefies

Background of Standard Evolution

As the first part of the SN/T 5366 series, this standard innovatively incorporates the commercial kit detection method into the entry-exit inspection and quarantine system, making up for the efficiency bottleneck of the traditional culture method. The test piece technology shortens the detection time to within 26 hours through the prefabricated culture medium system.

More samples: DL/T 368-2010 GB 18306-2015 GB 50021-2001(2009)

Sample only — not a preview of SN/T 5366.1-2022

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