WS/T 792-2021 in English
VALIDDetection standard of antibody against Schistosoma japonicum - Enzyme-linked immunosorbent assay
- Issued on:2021-11-23
- Implemented on:2022-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$204.00
Introduction
Technical background and evolution of the standard
This standard, as a supplementary refinement of WS 261-2006, is the first to establish a special technical specification for enzyme-linked immunosorbent assay. Compared with traditional pathogen detection, ELISA method increases the sensitivity from 60% to more than 90%, which is particularly suitable for large-scale screening scenarios.
Analysis of core detection process
| Steps | Key parameters | Key points of quality control |
|---|---|---|
| Antigen coating | 1:1000-1:3000 dilution | 4℃ overnight≥16h |
| Serum incubation | 1:100 dilution | 37℃±1℃ water bath |
| Enzyme labeling reaction | SPA-HRP 1:4000 | Avoid light operation |
Specifications for preparation of key reagents
Soluble egg antigen preparation requires three freeze-thaw cycles, and the protein concentration should be controlled at 0.75mg/mL±5% after centrifugation at 10,000g. Clinical practice shows that the difference between antigen batches needs to be monitored by the OD value ratio (P/N) of positive and negative controls, and the qualified range is 2.1-3.5.
Implementation suggestions and common problems
1. Pipette calibration: The 100μL range error should be verified by weighing method every month
2. Inter-plate variation control: The same batch of samples should be dispersed in different ELISA plates for detection
3. Critical value processing: When the P/N value is 1.8-2.1, it should be retested or combined with clinical evaluation
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