Methods for protein separation and purification

Methods for protein separation and purification, Total:188 items.

The international standard classification for "Methods for protein separation and purification" includes: Man-made fibres , food products in general , Animal feeding stuffs , Latex and raw rubber , Fruits. Vegetables , Edible oils and fats. Oilseeds , Biology. Botany. Zoology , General methods of tests and analysis for food products , Protection against crime .

The Chinese standard classification for "Methods for protein separation and purification" includes: Chemical fibre in general , Food sanitation , Basic standards and general methods , Livestock and poultry feed and additive , Medical and foodstuff rubber products , Basic standard and general method on forestry , Other cash crops , Oil crop and product , Melon, fruit and vegetable planting and product , Environmental Condition and General Test Method , Basic standards and general methods , Crime judging technology , Cereal crop and forage crop in general .

National Metrological Technical Specifications of the People's Republic of China

JJF 2204-2025 Protein Purification Analyzer Calibration Specifications

Group Standards of the People's Republic of China

T/ZGKSL 009-2023 Test method for determination of collagen secreted from human cells induced by recombinant collagen
T/SHNX 001-2025 Determination of lactoferrin, α-lactalbumin and β-lactoglobulin in raw bovine milk by liquid chromatography-tandem mass spectrometry
T/CASME 378-2023 Technical specifications for the isolation and purification of lactobacillus curvatus protease

Gansu Provincial Standard of the People's Republic of China

DB62/T 2277-2012 Alfalfa Leaf Protein Separation and Extraction Technical Regulations

Professional Standard - Chemical Industry

HG/T 3645-1999 Method for the determination of aqueous extractable protein in natural rubber gloves using the Lowry method

Professional Standard - Textile

FZ/T 50018-2013 Determination of the protein content of the protein viscose fiber

General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China

GB/T 8856-1988 Method for determination of crude protein in fruit and vegetable products
GB/T 14489.2-1993 Method for determination of crude protein in oilseed
GB/T 35809-2018 Method for analysis of forestry biomass—Determination of protein content
GB/T 17811-2008 Determination of pepsin digestibility in animal protein feeds - Filtration method
GB/T 21870-2008 Medical gloves made from natural rubber latex - Determination of water-extractable protein using the modified Lowry method
GB 8856-1988 Method for Determination of Crude Protein in Fruit and Vegetable Products
GB/T 17811-2025 Determination of pepsin digestibility for animal-derived protein feeds—Filtration method
GB/T 34800-2017 Detection method of activity and impurity of proteinase K
GB/T 15673-1995 Method for determination of crude protein in edible fungi
GB/T 5009.5-1985 Method for determination of protein in foods
GB/T 14771-1993 Method for determination of protein content in foods
GB/T 17811-1999 Determination of digestibility in animal protein feeds--Pepsin method
GB/T 19495.8-2004 Detection of genetically modified plants and derived products-Protein based methods

Professional Standard - Agriculture

NY/T 449-2001 Identification purity of maize variety using salting in protein eletrohporesis

Hebei Provincial Standard of the People's Republic of China

DB13/T 221-1994 Method for testing the purity of corn hybrids, inbred lines, and wheat seeds - protein electrophoresis

Professional Standard - Public Safety Standards

GA/T 819-2009 Analytical method of acid dyes on the protein fibers

Jilin Provincial Standard of the People's Republic of China

DB22/T 294-2020 Method for identification of adulteration of crude protein in corn gluten meal

Professional Standard - Commodity Inspection

SN/T 0800.3-1999 Cereals and feedstuffs for import and export - Method for the determination of crude protein
SN/T 0803.9-1999 Oil bearing materials for import and export—Method for the determination of crude protein

British Standards Institution (BSI)

BS EN ISO 8968-4:2016 Milk and milk products. Determination of nitrogen content. Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)
BS ISO 5547:2008 Caseins - Determination of free acidity (Reference method)
BS EN ISO 8968-4:2016(2017) Milk and milk products — Determination of nitrogen content Part 4 : Determination of protein and non - protein nitrogen content and true protein content calculation (Reference method)
BS ISO 5545:2008 Rennet caseins and caseinates - Determination of ash (Reference method)
BS EN ISO 21572:2013 Foodstuffs. Molecular biomarker analysis. Protein-based methods
BS 6248-9:1984 Caseins and caseinates-Method for the determination of scorched particles
BS ISO 5546:2010 Caseins and caseinates - Determination of pH (Reference method)

Japanese Industrial Standards Committee (JISC)

JIS K 0602:2000 Proteins -- Serum albumin -- Methods for quantitative analysis
JIS K 0605:2000 Proteins -- Trypsin -- Methods for quantitative analysis
JIS K 0606:2000 Proteins -- Transferrin -- Methods for quantitative analysis
JIS K 0603:2000 Proteins -- Immunoglobulin -- Methods for quantitative analysis
JIS K 0604:2000 Proteins -- Monoclonal IgG -- Methods for quantitative analysis
JIS K 0607:2000 Proteins -- Glucoseoxydase -- Methods for quantitative analysis
JIS K 0602:1992 Methods for quantitative analysis of serum albumin
JIS K 0605:1992 Methods for quantitative analysis of trypsin
JIS K 0606:1993 Methods for quantitative analysis of transferrin

International Organization for Standardization (ISO)

ISO 8968-4:2016 Milk and milk products - Determination of nitrogen content - Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)
ISO 5549:1978 Caseins and caseinates; Determination of protein content (Reference method)
ISO 5547:2008 | IDF 91:2008 Caseins — Determination of free acidity (Reference method)
ISO 8968-4:2016 | IDF 20-4:2016 Milk and milk products — Determination of nitrogen content — Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)
ISO 5547:2008 Caseins - Determination of free acidity (Reference method)
ISO 5547:1978 Caseins; Determination of free acidity (Reference method)
ISO 5547:2008|IDF 91:2008 Caseins — Determination of free acidity (Reference method)
ISO 5546:2010 Caseins and caseinates - Determination of pH (Reference method)
ISO 5546:2010 | IDF 115:2010 Caseins and caseinates — Determination of pH (Reference method)
ISO 5546:2010|IDF 115:2010 Determination of pH value of casein and caseinate (standard method)

German Institute for Standardization

DIN EN ISO 8968-4 E:2013-09 Determination of nitrogen content of milk and milk products Part 4: Determination of protein and non-protein nitrogen content and calculation of pure protein content (Reference Method) (Draft)
DIN 10454:1983-03 Determination of protein content of caseins and caseinates; reference method
DIN EN ISO 8968-4:2016 Milk and milk products - Determination of nitrogen content - Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method) (ISO 8968-4:2016); German version EN ISO 8968-4:2016
DIN 10450:1983-03 Determination of free acidity of caseins; reference method
DIN EN ISO 8968-4:2016-09 Milk and milk products - Determination of nitrogen content - Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method) (ISO 8968-4:2016); German version EN ISO 8968-4:2016
DIN ISO 5550 E:2020-06 Caseins and caseinates - Determination of moisture content (Reference method)
DIN ISO 5550:2020-12 Caseins and caseinates - Determination of moisture content (Reference method) (ISO 5550:2006)
DIN 10453:1983-03 Determination of water content of caseins and caseinates; reference method
DIN 10452:1983-03 Determination of ash of rennet caseins and caseinates; reference method
DIN EN ISO 8968-4 E:2013 Draft Document - Milk and milk products - Determination of nitrogen content - Part 4: Determination of protein and non protein nitrogen content and true protein content calculation (Reference method) (ISO/DIS 8968-4:2013); German version prEN ISO 8968-...
DIN ISO 5545 E:2020-06 Rennet caseins and caseinates - Determination of ash (Reference method)
DIN 10466:2001 Determination of whey protein content in total protein of milk and milk products - Polarographic method
DIN 10456:1989-04 Determination of pH of caseins and caseinates; reference method
DIN ISO 5546 E:2020-08 Caseins and caseinates - Determination of pH (Reference method)
DIN SPEC 13290:2021 Protein analysis - Evaluation criteria for thermal unfolding methods (DSF and DSC); Text in German and English
DIN 10470:2011 Milk and milk products - Determination of whey protein content and casein content in total protein - Derivative spectroscopic method
DIN ISO 5545:2020-12 Rennet caseins and caseinates - Determination of ash (Reference method) (ISO 5545:2008)
DIN ISO 5550:2020 Caseins and caseinates - Determination of moisture content (Reference method) (ISO 5550:2006)
DIN 10470:1999 Determination of whey protein content and casein content in total protein of milk and milk products - Derivative spectroscopic method
DIN EN ISO 21572 E:2012-02 Protein-based methods for food molecular biomarker analysis (draft)
DIN EN ISO 21572 E:2019-03 Protein-based methods for food molecular biomarker analysis (draft)
DIN ISO 5545:2020 Rennet caseins and caseinates - Determination of ash (Reference method) (ISO 5545:2008)

Czech Standards Institute (UNMZ)

CSN 57 0111 Cast.10-1979 Testing methods for casein. Determination of purity
CSN 57 0111-5-2002 Testing methods for casein and caseinates - Determination of protein content
CSN 570111-5-2002 Testing methods for casein and caseinates - Determination of protein content
CSN 57 0111-2-2002 Testing methods for casein and caseinates - Methods of samphng of Chemical analysis
CSN 57 0111-3-2002 Testing methods for casein and caseinates - Determination of moisture
CSN 56 0186 Cast.12-1981 Testing methods for beer. art 12. etermination of proteins
CSN 46 7092-27-1998 Testing methods for feeding stuffs - Part 27: Determination of the protein content
CSN 56 0512-12-1995 Methods of milled produets analysis - Part 12: Determination of protein content
CSN 57 0111-1-2002 Testing methods for casein and caseinates - General regulations
CSN 57 0111-7-2002 Testing methods for casein and caseinates - Determination of ash
CSN 57 0111-12-2002 Testing methods for casein and caseinates - Determination of pH

Austrian Standards

ONORM DIN 10454-1993 Determination of protein content of caseins and caseinates - Reference method
ONORM DIN 10456-1993 Determination of pH of caseins and caseinates - Reference method

Gosstandart of Russia

GOST R 51468-1999 Caseins. Method for determination of free acidity
GOST R 57475-2017 Dried egg protein after extraction lysozyme. Specifications
GOST 25179-1990 Milk. Methods for determination of protein
GOST 34551-2019 Сonfectionery. Method for determination of mass fraction of protein
GOST R 51470-1999 Caseins and caseinates. Method for determination of protein content
GOST R 51466-1999 Caseins. Method for determination of "fixed ash" mass fraction
GOST 25179-2014 Milk and milk products. Method for determination of protein
GOST ISO 8968-4-2021 Milk and dairy products. Determination of nitrogen content. Part 4. Determination of protein and non-protein nitrogen content and calculation of true protein content (reference method)..
GOST ISO 5548-2016 Caseins and caseinates. Photometric method for determining the mass fraction of lactose
GOST R 51467-1999 Caseins and caseinates. Method for determination of pH
GOST 25011-1981 Meat and meat products. Methods of protein determination
GOST 25011-2017 Meat and meat products. Protein determination methods
GOST R 51463-1999 Rennet caseins and caseinates. Method for determination of ASH content
GOST ISO 5550-2017 Caseins and caseinates. Determination of moisture content (Reference method)
GOST R 51464-1999 Caseins and caseinates. Method for determination of water content
GOST R 51454-1999 Caseins and caseinates. Method of determination of nitrate and nitrite contents
GOST 13979.3-1968 Oilcakes oil-meals. Methods of determination of soluble proteins

Ente Nazionale Italiano di Unificazione

UNI 10414:1994 Soft wheat and durum wheat. Definition of varietal purity by electrophoresis of proteins. Rapid method.
UNI EN ISO 8968-4:2016 Milk and milk products - Determination of nitrogen content - Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)
UNI 22632:2002 Oilseeds - Vegetable proteins content - Determination of total proteins (biuret method)
UNI EN ISO 21572:2013 Foodstuffs - Molecular biomarker analysis - Protein-based methods

Spanish Association for Standardization (UNE)

UNE 34081:1975 ANALYSIS METHODS FOR CHOCOLATES. MILK PROTEINS.
UNE-EN ISO 8968-4:2016 Milk and milk products - Determination of nitrogen content - Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method) (ISO 8968-4:2016)
UNE 34080:1974 ANALYSIS METHODS FOR CHOCOLATES. DETERMINATION OF TOTAL NITROGEN. (PROTEINS).
AENOR UNE 34400-7:1967 Test methods for wheat flour. Determination of protein.
UNE 34400-7:1967 TEST METHODS OF WHEAT FLOUR. PROTEIN DETERMINATION
AENOR UNE-EN ISO 8968-4:2016 Milk and dairy products. Determination of nitrogen content. Part 4: Determination of protein and non-protein nitrogen content and calculation of true protein content (Reference method). (ISO 8968-4:2016)

American Society for Testing and Materials (ASTM)

ASTM D8238-18 Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins
ASTM F831-83 Net Protein Ratio (NPR) Test Method
ASTM D5712-99e1 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM D5712-05e1 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM D8238-24 Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins
ASTM D5712-10 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM D6188-17 Standard Test Method for Viscosity of Cellulose by Cuprammonium Ball Fall
ASTM D7818-12 Standard Test Method for Enumeration of Proteolytic Bacteria in Fresh (Uncured) Hides and Skins
ASTM D5712-15(2025) Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified Lowry Method
ASTM D5712-15(2020) Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified Lowry Method
ASTM D8238-18(2023) Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins

Standard Association of Australia （SAA）

AS 1871.9:1976 List of methods for examination of waters Nitrogen (albuminoid)
AS ISO 8968.4:2021 Milk and milk products — Determination of nitrogen content, Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)
AS N60:1970 Methods for the sampling and analysis of acid and rennet caseins

Indonesia Standards

SNI 14-1038-1989 Kertas dan karton, Cara uji kualitatip kasein dan protein kedelai
SNI 8074:2022 Flour extract of albumin from snakehead fish (Channa striata) as a source of albumin - Quality requirements and processing
SNI 14-1037-1989 Kertas dan karton, Cara uji lapisan salut berperekat protein

Korean Agency for Technology and Standards (KATS)

KS H ISO 5549:2006 Caseins and caseinates－Determination of protein content(Reference method)
KS H ISO 5549:2021 Caseins and caseinates — Determination of protein content(Reference method)
KS H 1204-2021 Testing method for crude proteins content
KS H ISO 5549-2021 Caseins and caseinates — Determination of protein content(Reference method)
KS H ISO 5549-2006(2016) Caseins and caseinates－Determination of protein content(Reference method)
KS H 1204-2012 Testing method for crude proteins content
KS H 1204-2001 Testing method for crude proteins content
KS H 1204-2022 Testing method for crude proteins content
KS J 4201-2008 Methods for quantitative analysis of serum albumin
KS J 4201-2023 Methods for quantitative analysis of serum albumin
KS H ISO 5547-2021 Caseins — Determination of free acidity(Reference method)
KS H ISO 5547-2006(2016) Caseins－Determination of free acidity(Reference method)
KS H ISO 5547:2006 Caseins－Determination of free acidity(Reference method)
KS H ISO 5547:2021 Caseins — Determination of free acidity(Reference method)
KS H ISO 5546:2006 Caseins and caseinates－Determination of pH(Reference method)
KS J 4201-2018 Methods for quantitative analysis of serum albumin
KS J 0023-2025 Biotechnology — Quantitative analysis method for proteins produced by biotechnology
KS J 4202-2008 Methods for quantitative analysis of immunoglobulin
KS H ISO 5550-2007(2021) Caseins and caseinates－Determination of moisture content(Reference method)

Swiss Association for Standardization (SNV)

SN 210 100-1987 Technical drawings - Sizes and layout of drawing sheets

Association Francaise de Normalisation

NF ISO 5547:2008 Caseins - Determination of free acidity (Reference method)
NF V04-221-4*NF EN ISO 8968-4:2016 Milk and milk products - Determination of nitrogen content - Part 4 : determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)
NF ISO 5550:2007 Caseins and caseinates - Determination of moisture content (reference method)
NF V04-387:1985 Caseins and caseinates. Determination of protein content (reference method).
NF V04-386:1985 Caseins. Determination of free acidity (reference method).
NF EN ISO 8968-4:2016 Milk and dairy products - Determination of nitrogen content - Part 4: determination of protein and non-protein nitrogen content and calculation of true protein content (Reference method)
NF V04-386*NF ISO 5547:2008 Caseins - Determination of free acidity (Reference method)
NF V03-021:2013 Foodstuffs - Molecular biomarker analysis - Protein-based methods
NF ISO 5545:2008 Rennet caseins and caseinates - Determination of ash (Reference method)
NF ISO 5546:2010 Caseins and caseinates - Determination of pH (Reference method)

Bureau of Indian Standards

IS 11964-1987 Method for determination of free acidity in casein (reference method)
IS 11964-2012 Caseins —— Determination of Free Acidity (Reference Method)
IS 7481-1974 How to measure protein efficiency ratio (PER)
IS 18836-2024 Milk and Milk Products - Determination of Nitrogen Content - Determination of Protein and Non-Protein Nitrogen Content and True Protein Content Calculation (Reference Method)
IS 2006-1988 Methods for the quantitative chemical analysis of binary mixtures of protein fibers and certain other non-protein fibers
IS 11962-1987 Method for determination of ash content in chymosin casein and caseinate (reference method)
IS 7219-1973 Methods for determination of protein in food and feed
IS 11920-2012 Cassein and Caseinates -Determination of Moisture Content (Reference Method)
IS 11962-2012 Rennet Caseins and Caseinates—— Determination of Ash (Reference Method)

International Dairy Federation (IDF)

IDF 91-2008 Caseins - Determination of free acidity (Reference method)
IDF 90-2008 Rennet caseins and caseinates - Determination of ash (Reference method)
IDF 78-2006 Caseins and caseinates. Determination of moisture content (Reference method)

Danish Standards Foundation

DANSK DS/EN ISO 8968-4:2016 Milk and milk products – Determination of nitrogen content – Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)
DANSK DS/ISO 21572:2019 Foodstuffs – Molecular biomarker analysis – Protein-based methods
DS/EN ISO 21572:2013 Foodstuffs - Molecular biomarker analysis - Protein-based methods

Lithuanian Standards Office

LST ISO 5549:2001 Caseins and caseinates. Determination of protein content (Reference method) (identical ISO 5549:1978)
LST ISO 5547:2008 Caseins - Determination of free acidity (Reference method) (ISO 5547:2008, identical)

National Standardisation Body (ASRO)

STAS SR 13348-1-1996 Tea. Analysis methods. Determination of proteic nitrogen by the Kjeldahl method. Spectrophotometric method

European Committee for Standardization (CEN)

EN ISO 21572:2013 Foodstuffs - Molecular biomarker analysis - Protein-based methods
EN ISO 21572:2019 Foodstuffs - Molecular biomarker analysis - Immunochemical methods for the detection and quantification of proteins (ISO 21572:2019)
EN ISO 8968-4:2001 Milk - Determination of Nitrogen Content - Part 4: Determination of Non-Protein Nitrogen Content

Standards Norway

NS-EN ISO 8968-4:2016 Milk and milk products — Determination of nitrogen content — Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method) (ISO 8968-4:2016)

Kingdom of Bahrain Testing and Metrology Directorate

BH GSO ISO 8968-4:2022 Milk and milk products — Determination of nitrogen content — Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)

GCC Standardization Organization

GSO ISO 8968-4:2021 Milk and milk products — Determination of nitrogen content — Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method)

Swedish Institute for Standards

SS-EN ISO 8968-4:2017 Milk and milk products - Determination of nitrogen content - Part 4: Determination of protein and non-protein nitrogen content and true protein content calculation (Reference method) (ISO 8968-4:2016)

COMMISSION FOR THE STANDARDS, METROLOGY AND QUALITY OF VIETNAM (STAMEQ)

TCVN 3705-1990 Aquatic products.Method for the determination of total nitrogen and protein content

Netherlands Institute for Standardization

NEN 3145-1966 Feeding stuffs. Methodsof analysis. Determination of crude protein

Others: Four ways to separate proteins