GB/T 34800-2017 in English

VALID

Detection method of activity and impurity of proteinase K

Issued on:2017-11-01
Implemented on:2018-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

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Standard No:	GB/T 34800-2017
Document status:	VALID
Title in English:	Detection method of activity and impurity of proteinase K
Title in Chinese:	蛋白酶K酶活力及杂质检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-11-01
Implemented on:	2018-05-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A21-Environmental Condition and General Test Method
Professional Classification:	GB-National Standard
Related Keywords:	impurity detection method detection method impurity detection methods proteinase k enzyme activity detection methods
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Enzyme activity screening method Determination of protein standard curve How proteases break down proteins How to develop protein color Protein size prediction methods Protein purification technology protein interaction method Polyprotein activity Enzyme liquid extraction and determination methods Plasminogen detection method Common methods for protein iodine labeling What are the methods for protein precipitation? Cell telomerase activity detection method Inclusion body protein renaturation method Bromelain detection method What are the methods for protein purification? Common methods for protein purification How to measure enzyme activity? Protein desalting methods and principles Methods for protein purification Leaf photosynthetic enzyme activity assay Ninhydrin method for protein detection UV detection of unknown proteins Enzyme extraction method Single impurity test method Protein storage solution preparation method Protein desalting methods and principles What are the methods for measuring enzyme activity? What are the methods for enzyme activity determination? Methods for determining enzyme activity Folin phenol protein determination method Protein detection methods for high school Commonly used methods for protein crystallization Methods for detecting enzyme activity Methods for detecting enzyme activity Protease Country Protein hydrolysis method Methods for detecting alpha-fetoprotein Protein detection methods Transferrin detection method Methods for measuring protein molecular weight Basic Methods for Determining Protein Disulfide Bonds Common methods for protein precipitation include Protein structure detection methods Protein wash purification method Detection methods of high molecular weight proteins Pectinase activity determination method Methods for detecting enzyme activity

《GB/T 34800-2017蛋白酶K酶活力及杂质检测方法》由424-cnis（中国标准化研究院）归口，主管部门为国家市场监督管理总局。

Introduction

National Standard of the People's Republic of China GB/T 34800—2017 Proteinase K Enzyme Activity and Impurity Detection Method

1. Standard Overview

This standard specifies the enzyme activity and impurity detection methods of proteinase K, which is applicable to the production and quality control of biochemical reagent products. This standard was proposed and coordinated by the China National Institute of Standardization, and the drafting units include many universities, scientific research institutions and enterprises.

2. Background of Standard Formulation

Proteinase K is an important biochemical reagent and is widely used in the field of biotechnology. With the expansion of its application scope, the requirements for product quality and detection methods are also increasing. The formulation of this standard aims to standardize the detection process and ensure the consistency and reliability of the product.

3. Detection Principle and Method

Proteinase K hydrolyzes bovine hemoglobin by catalyzing the cleavage of peptide bonds to produce phenolic amino acids. Under alkaline conditions, Folin's reagent reduces it to molybdenum blue and tungsten blue, and the absorbance change is proportional to the tyrosine content, thereby calculating the enzyme activity.

4. Comparison table of standard frameworks

The following table compares the standard frameworks for detection methods of proteinase K and other related enzymes (such as RNase and DNase):

Enzyme name	Detection principle	Scope of application	Reference standard
Protease K	Peptide bond cleavage and Folin reagent colorimetry	Production and detection of biochemical reagents	GB/T 34800—2017
RNase	RNA hydrolysis and phosphomolybdic acid reduction	Detection of biochemical reagents	GB/T 34222
DNase	DNA hydrolysis and colorimetric determination	Detection of biochemical reagents	GB/T 34801

5. Implementation suggestions

To ensure accurate test results, it is recommended to pay attention to the following points when implementing this standard:

Instrument calibration: Use a constant temperature water bath, pH meter and spectrophotometer that meet the accuracy requirements.
Reagent preparation: Folin reagent must be prepared strictly according to the ratio and stored away from light.
Operation specifications: Strictly abide by the reaction time and temperature conditions to ensure the reliability of experimental data.

More samples: GB 18306-2015 YD/T 3934-2021 JC/T 2453-2018

Sample only — not a preview of GB/T 34800-2017

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