GB/T 38475-2020 in English

Analysis of Standard Technical Framework

Core Detection Principle

Based on the antigen-antibody competitive binding reaction, the biological toxins in the sample are combined with the labeled antibodies on the colloidal gold immunochromatographic test strips, and the color intensity of the test line (T line) is measured by the reader. The four-parameter logistic curve fitting algorithm is used for quantification, and the standard curve R²≥0.99 is required.

Key Operation Points

Implementation recommendations

1. It is recommended to establish a library of standard substances, including 4 standards such as aflatoxin B1
2. The vortex oscillator and centrifuge need to be calibrated before testing
3. Each batch of testing should include a negative control and 3 concentration level quality control samples

Technology evolution analysis

Compared with the traditional HPLC method, the colloidal gold method shortens the detection time from 4 hours to 15 minutes, but attention should be paid to the matrix interference that may be caused by pigments such as red yeast rice. This standard clarifies the specific parameters of purification for the first time.