GB/T 38478-2021 in English

VALID

Determination of astaxanthin opticalisomers content—Liquid chromatography

Issued on:2021-12-31
Implemented on:2022-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $150.00
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Standard No:	GB/T 38478-2021
Document status:	VALID
Title in English:	Determination of astaxanthin opticalisomers content—Liquid chromatography
Title in Chinese:	虾青素旋光异构体含量的测定液相色谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2021-12-31
Implemented on:	2022-07-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A21-Environmental Condition and General Test Method
Professional Classification:	GB-National Standard
Related Keywords:	content liquid chromatography introduction analysis high performance liquid chromatography astaxanthin opticalisomers astaxanthin oil test astaxanthin implementation suggestions case
Related Topics:	liquid phase optical rotation Isomers MS isomers isomer isomer method Detection of isomers liquid phase multispectral γ isomer Isomer content Solid Optical Rotation liquid spectrometer Chromatographic element content Optical element How to detect isomers liquid phase optical rotation Isomer detection Isomers spectrometer liquid Isomer detection Element content spectrum Pigment content detection liquid spectrometer Spectrometers measure liquids Isomer+ Gas Chromatography Isomers Phytochrome spectrum Liquid phase and spectroscopy Isomer content LC sample volume Liquid phase content peak Isomers liquid phase liquid spectrometry Optical content spectral liquid liquid element content Isomer Gas Chromatography Isomers Optical rotation of liquid phase spectral elements semi-quantitative liquid phase liquid chromatography in english spectrometer liquid Abnormal ignition of gas chromatography Detection of isomers Optical Spectroscopy Chromatographic secretion Chromatographic isomers Photochromatography LC content isomer chromatography isomer ms LC content Gas Spectroscopy test isomer Optical rotator structure Astaxanthin detection All pigments Optical rotation of liquid phase Optical chromatography Separation of isomers and non-isomers liquid stratified content shrimp field Determination method of liquid phase content Chromatography detection content Quaternary liquid chromatography Spectrum Three Elements Solid Optical Rotation Optic Isomer melting point Pigment liquid phase method Isomer Chromatographic Peaks Optical rotator structure Pigment content detection Measurement of Optical Rotation Astaxanthin Mass Spec + Isomers LC content c icp spectroscopic liquid phase Monochromator for spectral measurements Liquid Optical Rotation ms+ isomer liquid element content Astaxanthin detection Method for Determination of Pigment Content Optical Spectroscopy Gas Chromatography Isomers Chromatographic helix Three-color rotation Liquid Chromatography Main Measurements Liquid phase relative quantification Is the content determined by polarimetry? optically active liquid What are the abnormal peaks of gas chromatography single point quantification Optical rotation quantification Liquid Chromatography + Assay Isomer specific rotation Isomer Chromatography Determination of Astaxanthin in Haematococcus by Liquid Chromatography Isomer peak Determination of content by polarimetry Liquid phase test Pigment Content Spectrum solution liquid phase Measuring liquid Determination of isomers by specific rotation Spectrometers measure liquids Optical content Isomer content specific rotation isomers How is isomer content tested? polarimetry multispectral optical rotation Measuring liquid Spectrometers for measuring liquids light content Normal Phase Chromatography Isomers Applications of polarimetry Measurement of optical rotation properties Astaxanthin 5 Optical structure Liquid Chromatography Measuring Range Astaxanthin' GBT38478 GB/T 38478-2021 Optical Chromatography LC content liquid chromatography optical rotation Measure pigment content Analysis and treatment of abnormal peaks in liquid chromatography Astaxanthin coloring effect Analysis of concentration results of polarimeter measurement of optically active liquids Determination of choline content by liquid chromatography rotamer isomer conversion isomer liquid phase liquid phase quantitative method Preparation of Diastereomers Normal phase chromatography isomer method development Methods for isomer conversion Liquid phase content standard curve Astaxanthin Europe Liquid phase method for determination of cadmium content Astaxanthin industry Isomer detection and determination Determination method of photosynthetic pigment content in ethanol Determination method of total anthocyanin content Liquid phase analysis method for cis-trans isomers Astaxanthin quality Normal phase isomer method establishment The significance of liquid phase qualitative and quantitative methods Stereoisomer Analysis Methods Total anthocyanin content detection method Method for testing content using liquid chromatography Cis-trans isomer liquid phase analysis method Limit of quantitation for liquid phase methods Optical rotation measurement solution preparation method Isomer limits How to measure anthocyanin content Commonly used liquid phase methods for separating isomers Pigment content determination method Astaxanthin Design

《GB/T 38478-2021虾青素旋光异构体含量的测定液相色谱法》由424-cnis（中国标准化研究院）归口，主管部门为国家市场监督管理总局。

Introduction

Analysis of standard technical framework

Technical elements	GB/T38478-2021	Internationally commonly used methods	Innovation points
Chromatographic column type	Tris(3,5-dichlorophenylcarbamate) cellulose chiral column	Amylose derivative chiral column	Improve the separation of 3R,3'S configuration
Enzymatic hydrolysis conditions	Reaction at 37℃ for 60min	Reaction at 25℃ for 120min	Time shortened by 50%
Limit of quantitation	500mg/kg	1000mg/kg	Sensitivity increased by 1 times

Key operating points

High performance liquid chromatography Special attention should be paid to:

The mobile phase ratio is strictly controlled at methyl tert-butyl ether: acetonitrile = 35:65 (v/v)
The column temperature is maintained at 25±0.5℃. Temperature fluctuations will cause retention time shift
The detection wavelength of 470nm corresponds to the maximum absorption peak of astaxanthin

Implementation suggestions

Case: An astaxanthin oil test showed a forward peak. After investigation, the following results were found:

Incomplete enzymatic hydrolysis: add 50μL cholesterol esterase working solution
Insufficient petroleum ether extraction: increase to 3 extractions
Nitrogen blowing temperature is too high: control below 40℃

Technology Evolution Analysis

The main improvements of this standard compared with ASTMD6868 are:

Introduction of cholesterol esterase directional hydrolysis technology
Optimization of mobile phase system to improve peak symmetry
Establishment of -20℃ light-proof storage specification

More samples: JGJ 94-2008 GB 18306-2015 DL/T 368-2010

Sample only — not a preview of GB/T 38478-2021

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