GB/T 35809-2018 in English

VALID

Method for analysis of forestry biomass—Determination of protein content

Issued on:2018-02-06
Implemented on:2018-09-01
File Format:PDF
Delivery:Via email within 1~3 business days

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Standard No:	GB/T 35809-2018
Document status:	VALID
Title in English:	Method for analysis of forestry biomass—Determination of protein content
Title in Chinese:	林业生物质原料分析方法蛋白质含量测定
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-02-06
Implemented on:	2018-09-01
Chinese Classification:	B60-Basic standard and general method on forestry
Professional Classification:	GB-National Standard
Related Keywords:	disadvantages kjeldahl method protein forestry biomass determination kjeldahl method kjeldahl nitrogen analyzer protein content dumas combustion method
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《GB/T 35809-2018林业生物质原料分析方法蛋白质含量测定》由TC416（全国林业生物质材料标准化技术委员会）归口，主管部门为国家林业和草原局。

Introduction

Interpretation of GB/T 35809—2018 National Standard of the People's Republic of China

Method name	Principle overview	Scope of application	Advantages and disadvantages
Kjeldahl method	Protein is decomposed by catalytic heating to generate ammonia and its content is determined by chemical reaction.	Applicable to the determination of protein content in various forestry biomass raw materials.	Advantages:Classic method with accurate results; Disadvantages:Complicated operation and time-consuming.
Dumas combustion method	By burning the sample at high temperature, the nitrogen content is determined using a thermal conductivity detector.	Suitable for rapid batch detection of protein content in forestry biomass raw materials.	Advantages:High efficiency and speed, suitable for large batches of samples; Disadvantages:High equipment cost.

Standard formulation background and technology evolution analysis

With the increasing global demand for renewable energy and environmentally friendly materials, the development and utilization of forestry biomass raw materials has become a hot topic. As an important nutrient in biomass, the determination of protein content is of great significance for evaluating raw material quality and guiding processing technology.

Technical details of Kjeldahl method

Kjeldahl nitrogen analyzer is the core equipment of this method. The operation steps include sample treatment, acid hydrolysis, distillation and titration. Among them, the use of key reagents such as copper sulfate, potassium sulfate and indicators directly affects the accuracy of the determination results.

Technical features of Dumas combustion method

Dumas nitrogen analyzer decomposes protein into nitrogen through high-temperature combustion and uses a thermal conductivity detector for quantitative analysis. The advantage of this method is that it is fast and efficient, and it is particularly suitable for scenarios that require a large number of samples to be tested.

Implementation suggestions

Choose the appropriate method according to the experimental requirements: Kjeldahl nitrogen determination is suitable for high-precision single sample analysis, while Dumas combustion method is suitable for large-scale rapid testing.
Ensure that the equipment calibration and reagent preparation are accurate.
It is recommended to have professional personnel to operate, especially when using hazardous chemicals and high-temperature equipment.
Establish a data recording and analysis system to ensure the long-term traceability of experimental results.

More samples: JGJ 94-2008 YD/T 3934-2021 DL/T 5154-2012

Sample only — not a preview of GB/T 35809-2018

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