GB/T 35808-2018 in English
VALIDMethod for analysis of forestry biomass-Determination of cellulase activity
- Issued on:2018-02-06
- Implemented on:2018-09-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$97.00
《GB/T 35808-2018林业生物质原料分析方法 纤维素酶活性测定》由TC416(全国林业生物质材料标准化技术委员会)归口,主管部门为国家林业和草原局。
Introduction
1. Background and significance of standard formulation
With the increasing global demand for renewable energy, the efficient use of forestry biomass as an important green energy and industrial raw material has attracted much attention. Cellulase is a key tool for decomposing cellulose, and its activity determination is of great significance for evaluating the quality and suitability of biomass raw materials. The formulation of this standard fills the gap in this field in China and provides technical support for the development of the forestry biomass industry.
2. Comparative analysis of standard frameworks
| Standard dimensions | GB/T 35808—2018 | International similar standards | Industry reference standards |
|---|---|---|---|
| Scope of application | Determination of cellulase activity for analysis of forestry biomass raw materials | Mainly used for industrial biomass raw materials | Focus on the enzymatic hydrolysis efficiency of agricultural residues |
| Determination principle | Based on the color change of the reaction between reducing sugar and 3,5-dinitrosalicylic acid | Using fluorescent labeling technology | Relying on enzyme activity kinetic analysis |
| Operation steps | Includes sample preparation, standard curve drawing and result calculation | Involves high temperature and high pressure treatment | Focus on analysis of enzymatic hydrolysis kinetic parameters |
3. Implementation suggestions and precautions
Case study: Practical application of cellulase activity determination
- A forestry enterprise used this standard to determine the cellulase activity of different types of biomass raw materials, and found that coniferous wood raw materials had a higher enzymatic hydrolysis efficiency.
- It is recommended that enterprises pay attention to controlling the reaction temperature and pH value in actual operations to ensure the accuracy of the determination.

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