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viral haemorrhagic septicaemia viral hemorrhagic septicemia viral antigens aquatic disease diagnosis technology rapid diagnosis macao special administrative region scopethis standard tank supply storage tank figure heat-shrinkable epdm tubing
GB/T 15805.3-2018 in English

GB/T 15805.3-2018 in English

VALID

Code of diagnosis for viral haemorrhagic septicaemia(VHS)

  • Issued on:2018-07-13
  • Implemented on:2019-02-01
  • File Format:PDF
  • Delivery:Via email within 1~3 business days
Price(USD): $250.00
$243.00
Standard No: GB/T 15805.3-2018
Document status: VALID
Title in English: Code of diagnosis for viral haemorrhagic septicaemia(VHS)
Title in Chinese: 病毒性出血性败血症诊断规程
Language: English
File Format: Electronic (PDF)
Delivery: Via email within 1~3 business days
Issued on: 2018-07-13
Implemented on: 2019-02-01
Superseding: GB/T 15805.3-2008 Quarantine methods of fish - Part 3: Viral hemorrhagic septicemia virus(VHSV)
ICS Classification: 65.020.30-Animal husbandry and breeding
Chinese Classification: B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification: GB-National Standard
Related Keywords: viral haemorrhagic septicaemia
viral hemorrhagic septicemia
viral antigens
aquatic disease diagnosis technology
rapid diagnosis
Related Topics: positive negative
Viral
virus+
blood virus
virus standard
batch sepsis
septicemia
diagnostic regulations
Vascular Diagnosis System
Vascular Diagnosis System
blood disease
diagnostic serum
scattered disease
blood for diagnosis
Serum Prion
sepsis diagnosis
Virus
blood disease second child
virus security
Glutaremia
Prion serum
anemia
anemia
fruit bat hemorrhagic fever
Prion serum
failure syndrome
blood coagulation
pseudoleukemia
blood toxicity
blood diagnostic kit
positive and negative
plasmaserumfor in vitro diagnostic
"septicemia
Early diagnosis of leukemia
Diagnosis of various types of leukemia
How Leukemia Is Diagnosed
Diagnosis of Leukemia Gold
blockade
Acute leukemia
pseudoleukemia
viral protein virus
GB/T 15805.3
GB/T 15805.3-2008
GB/T 15805.3-2018
Diagnostic formulas for blood diseases
Medical records of ischemic cerebrovascular disease
Plasma indications
Determine gas toxicity
Diagnosis of acquired von Willebrand disease
Diagnosis of disseminated intravascular coagulation
sepsis diagnosis
Diagnosis of anemic heart disease
Diagnosis of hemolytic uremic syndrome
Blood bromide diagnostic method
Diagnosis of beta hemoglobinopathy
Diagnosis of vascular calcification
Kikuchi disease diagnosis
Classification and diagnosis of coagulopathy
Diagnosis of hypoglycemia
Methemoglobinemia Diagnosis
Diagnosis of Nephrogenic Diabetes Insipidus
Diagnosis of hypocalcemia
Diagnosis of Hyperphosphatemia
Diagnosis of acute schistosomiasis
Diagnosis of hypochloremia in children
Diagnosis of Hemorrhagic Disease of the Newborn
Diagnosis of hemothorax

《GB/T 15805.3-2018病毒性出血性败血症诊断规程》由TC156(全国水产标准化技术委员会)归口,TC156SC11(全国水产标准化技术委员会水产养殖病害防治分会)执行,主管部门为农业农村部。


Introduction

GB/T 15805.3—2018 Standard Overview

This standard is an important specification in the field of aquaculture, mainly for the detailed provisions of the diagnostic method of viral hemorrhagic septicemia (VHS). Compared with the old version of GB/T 15805.3—2008, the new version of the standard adds sampling, clinical symptom description and a variety of detection techniques, including indirect immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and RT-PCR.

Standard formulation background and technology evolution analysis

1. Standard revision background

Viral hemorrhagic septicemia (VHS) is a fish disease with high mortality, posing a serious threat to the global aquaculture industry. Although the original standard GB/T 15805.3-2008 standardized the detection method to a certain extent, with the in-depth research on pathogens and the advancement of technology, a new version of the standard came into being.

2. Technological evolution

  • Increase sampling and clinical symptom description
  • Introduce RT-PCR and fluorescent RT-PCR technology
  • Optimize virus isolation and identification process
  • Add comprehensive judgment criteria

Comparative analysis of diagnostic methods

Detection method Technical features Scope of application Sensitivity Required equipment
Indirect immunofluorescence antibody test (IFAT) Use labeled antibodies to detect viral antigens, and the results are intuitive. Applicable to isolated and cultured positive cells. High Fluorescence microscope
Enzyme-linked immunosorbent assay (ELISA) Detects viruses through antigen-antibody reactions and quantifies results. Suitable for large-scale sample screening. Medium Microplate reader
RT-PCR Amplifies virus-specific nucleic acids for rapid diagnosis. Suitable for early diagnosis and molecular epidemiology studies. High PCR instrument

Implementation suggestions

1. Scope of implementation

This standard is applicable to the quarantine of various types of aquaculture and wild fish, and plays an important role in disease prevention and control.

2. Personnel training

Operators must undergo professional training and be familiar with the operating procedures and quality control points of various testing technologies.

3. Equipment and reagent management

The laboratory should be equipped with necessary instruments and equipment and calibrated regularly; reagents and consumables must meet the requirements of the standard to ensure the accuracy of the test results.


Conclusion

The release of GB/T 15805.3—2018 marks an important progress in my country's aquatic disease diagnosis technology. By comprehensively using a variety of detection methods, the diagnostic ability of viral hemorrhagic septicemia can be effectively improved, providing strong support for the healthy development of the aquaculture industry.

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