GB/T 15805.3-2018 in English
VALIDCode of diagnosis for viral haemorrhagic septicaemia(VHS)
- Issued on:2018-07-13
- Implemented on:2019-02-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$243.00
《GB/T 15805.3-2018病毒性出血性败血症诊断规程》由TC156(全国水产标准化技术委员会)归口,TC156SC11(全国水产标准化技术委员会水产养殖病害防治分会)执行,主管部门为农业农村部。
Introduction
GB/T 15805.3—2018 Standard Overview
This standard is an important specification in the field of aquaculture, mainly for the detailed provisions of the diagnostic method of viral hemorrhagic septicemia (VHS). Compared with the old version of GB/T 15805.3—2008, the new version of the standard adds sampling, clinical symptom description and a variety of detection techniques, including indirect immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and RT-PCR.
Standard formulation background and technology evolution analysis
1. Standard revision background
Viral hemorrhagic septicemia (VHS) is a fish disease with high mortality, posing a serious threat to the global aquaculture industry. Although the original standard GB/T 15805.3-2008 standardized the detection method to a certain extent, with the in-depth research on pathogens and the advancement of technology, a new version of the standard came into being.
2. Technological evolution
- Increase sampling and clinical symptom description
- Introduce RT-PCR and fluorescent RT-PCR technology
- Optimize virus isolation and identification process
- Add comprehensive judgment criteria
Comparative analysis of diagnostic methods
| Detection method | Technical features | Scope of application | Sensitivity | Required equipment |
|---|---|---|---|---|
| Indirect immunofluorescence antibody test (IFAT) | Use labeled antibodies to detect viral antigens, and the results are intuitive. | Applicable to isolated and cultured positive cells. | High | Fluorescence microscope |
| Enzyme-linked immunosorbent assay (ELISA) | Detects viruses through antigen-antibody reactions and quantifies results. | Suitable for large-scale sample screening. | Medium | Microplate reader |
| RT-PCR | Amplifies virus-specific nucleic acids for rapid diagnosis. | Suitable for early diagnosis and molecular epidemiology studies. | High | PCR instrument |
Implementation suggestions
1. Scope of implementation
This standard is applicable to the quarantine of various types of aquaculture and wild fish, and plays an important role in disease prevention and control.
2. Personnel training
Operators must undergo professional training and be familiar with the operating procedures and quality control points of various testing technologies.
3. Equipment and reagent management
The laboratory should be equipped with necessary instruments and equipment and calibrated regularly; reagents and consumables must meet the requirements of the standard to ensure the accuracy of the test results.
Conclusion
The release of GB/T 15805.3—2018 marks an important progress in my country's aquatic disease diagnosis technology. By comprehensively using a variety of detection methods, the diagnostic ability of viral hemorrhagic septicemia can be effectively improved, providing strong support for the healthy development of the aquaculture industry.

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