GB/T 20760-2006 in English

《GB/T 20760-2006牛肌肉、肝、肾中的α-群勃龙、β-群勃龙残留量的测定 液相色谱-紫外检测法和液相色谱-串联质谱法》由469-nq（国家标准委农业食品部）归口，主管部门为国家标准化管理委员会。
本标准规定了牛肌肉、肝、肾中α-群勃龙、β-群勃龙残留量的液相色谱-紫外检测法和液相色谱-串联质谱测定方法。本标准适用于牛肌肉、肝、肾中α-群勃龙、β-群勃龙残留量的测定。本标准的方法检出限：α-群勃龙、β-群勃龙的液相色谱-紫外检测法和液相色谱-串联质谱的检出限均为2 μg/kg。

Method for the determination of α-trenbolone, β-trenbolone residues in bovine muscle, liver and kidney -
LC-UV method and LC-MS-MS method

1 Scope

This standard specifies the LC-UV method and LC-MS-MS method for the determination of α-trenbolone, β-trenbolone residues in bovine muscle, liver and kidney.

This standard is applicable to the determination of α-trenbolone, β-trenbolone residues in bovine muscle, liver and kidney.

For the method detection limit of this standard: the detection limits of LC-UV method and LC-MS-MS method for the determination of α-trenbolone, β-trenbolone are both 2μg/kg.

2 Normative references

The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.

GB/T 6379.1 Accuracy(trueness and precision)of measurement methods and results - Part 1: General principles and definitions (GB/T 6379.1-2004; ISO 5725-1: 1994, IDT)
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method (GB/T 6379.2-2004; ISO 5725-2:1994, IDT)
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-1992; neq ISO 3696:1987)

Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Apparatus
6 Preparation and preservation of specimen
7 Determination procedures
8 Result calculation
9 Precision
Annex A (Informative) Liquid chromatogram of reference-materials
Annex B (Informative) Recovery rate