GB/T 35917-2018 in English

VALID

Rapid detection for animal-derived materials—Membrane-based array method

Issued on:2018-02-06
Implemented on:2018-09-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $250.00
$243.00

Standard No:	GB/T 35917-2018
Document status:	VALID
Title in English:	Rapid detection for animal-derived materials—Membrane-based array method
Title in Chinese:	常见动物源性成分快速测定膜芯片法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-02-06
Implemented on:	2018-09-01
ICS Classification:	67.050-General methods of tests and analysis for food products
Chinese Classification:	X04-Basic standards and general methods
Professional Classification:	GB-National Standard
Related Keywords:	detection targets animal type detection gene application cases pig cox1 gene rapid screening rapid detection meat products cattle cox1 gene detection membrane-based array method introduction animal-derived materials
Related Topics:	chip animal origin Rapid detection of material composition Oxygen Fast Source Mass Spectrometry Wave plate fast orientation Animal Rapid Test Box Rapid Determination of Tetracycline Tablets Rapid Determination Method for Tetracycline Tablets GB/T 35917-2018 Fast film transfer instrument membrane probe ingredients of animal origin Chip Spotter animal origin ingredients Rapid Composition Analysis animal source animal ingredients Rapid Moisture Determination Rapid detection of food of animal origin ingredients of animal origin fast one sided Non-Animal Origin Ingredients How to test for ingredients of animal origin rapid eye movement source ingredient Common Analysis Methods active chip Fast film transfer Common Quantitative Analysis Methods Fast film transfer instrument ss rapid determination method Rapid Determination of Antimony rapid determination of cod Composition Standard Rapid Component Identification common food ingredients common analysis Chip test GBT35917 GB/T 35917-2018 CA724 chip method Film-forming performance measurement method Determination methods of common organic elements Common methods for nucleic acid quantitative determination include Common molecular hybridization methods are divided into Commonly used membrane separation methods Common pharmaceutical quantitative analysis methods Qualitative and rapid detection method for cyanide Film forming test method Common quantitative and qualitative analysis methods Rapid Moisture Determination Repeatability Chip foreign matter component analysis method Film forming speed test method

《GB/T 35917-2018常见动物源性成分快速测定膜芯片法》由424-cnis（中国标准化研究院）归口，主管部门为国家市场监督管理总局。

Introduction

1. Background and significance of the standard

GB/T 35917-2018 "Membrane chip method for rapid determination of common animal-derived ingredients" is an important national standard formulated by China for the detection of animal-derived ingredients in meat products and feed. The standard was drafted by the China Institute of Standardization and specifies the application of the membrane chip method in the detection of animal-derived ingredients.

2. Detection principle

The standard is based on polymerase chain reaction (PCR) technology and membrane chip hybridization technology. Primers are designed for specific animal genes, and after multiple PCR amplification, the amplified products are hybridized with specific probes fixed on the membrane chip.

3. Detection Targets

Animal Type	Detection Gene	Application Cases
Pig	cox1 Gene	Rapid Screening of Porcine Ingredients in Meat Products
Cattle	cox1 Gene	Detection of Foreign Animal Ingredients in Feed
Sheep	cytb Gene	Traceability management of the livestock industry

4. Testing steps and quality control

Sample preparation: Meat products and feed samples should be sampled according to GB/T9695.19 and GB/T14699.1 standards and processed by a high-throughput tissue grinder.

DNA extraction and purification: Use CTAB lysis buffer combined with phenol/chloroform method to extract genomic DNA to ensure purity (A260/A280 ratio between 1.7-2.1).

PCR amplification: Prepare multiplex PCR reaction system (see Table 3) and set reaction parameters (see Table 4).

5. Result determination and reporting

The membrane chip results can be observed by naked eyes or analyzed by a membrane chip reader. Positive signals are blue spots, while negative signals are colorless.

More samples: GB 50021-2001(2009) NB/T 47013.2-2015 QC/T 942-2021

Sample only — not a preview of GB/T 35917-2018

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