GB/T 37077-2018 in English

VALID

Determination of cholesterol in eggs-Enzymatic method

Issued on:2018-12-28
Implemented on:2019-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $100.00
$97.00

Standard No:	GB/T 37077-2018
Document status:	VALID
Title in English:	Determination of cholesterol in eggs-Enzymatic method
Title in Chinese:	禽蛋中胆固醇含量的测定酶法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-12-28
Implemented on:	2019-07-01
ICS Classification:	67.050-General methods of tests and analysis for food products
Chinese Classification:	X04-Basic standards and general methods
Professional Classification:	GB-National Standard
Related Keywords:	eggs-enzymatic method introduction gb/t cholesterol content enzymatic method extraction isolate cholesterol cholesterol esters
Related Topics:	enzyme cholesterol Cholesterol UV Detection Cholesterol UV cholesterol quality alcohol content poultry eggs Total Cholesterol Content Total Cholesterol Testing cholesterol hydroxylase cholesterol quality In biochemical/enzymatic assays high cholesterol Cholesterol 50 Method Cholesterol Content Method Enzyme content determination method Enzyme Content Determination Method China Poultry Federation Determination of water content in solids GB/T 37077-2018 cholesterol method Enzyme Content Determination Method poultry egg standard q3 cholesterol high cholesterol enzymatic tyrosol cholesterol esterase Determination method of enzyme content GBT37077 GB/T 37077-2018 Enzymatic method for serum total cholesterol trkb+cholesterol Cholesterol Aladdin Enzyme encapsulation method Solid content detection method What are the four methods of immobilizing enzymes? Enzyme immobilization method Characteristics of adsorption method among enzyme immobilization methods Cholesterol gas phase detection method Determination method of chymotrypsin content Determination of cholic acid pharmacopoeia Preparation method of immobilized enzyme Cholesterol testing method principle Methods for immobilizing enzymes Cholesterol liquid phase detection method Method for detecting ethanol content in solids Enzyme protein content determination method What omics method is used to measure enzyme content? Cholesterol measurement method Cholesterol Testing Methods What are the methods for immobilizing enzymes? Biochemical determination of steroids Preparation method of solid enzyme Method for preparing immobilized enzyme Common enzyme immobilization methods Several methods for preparing immobilized enzymes Paclitaxel content determination method Total cholesterol calculation method

《GB/T 37077-2018禽蛋中胆固醇含量的测定酶法》由TC274（全国畜牧业标准化技术委员会）归口，主管部门为农业农村部。

Introduction

GB/T 37077—2018 Standard Overview

This standard specifies the method for determining the cholesterol content in poultry eggs using an enzymatic method, which is applicable to a variety of fresh poultry eggs such as chicken eggs, duck eggs, and goose eggs. This method has the characteristics of high sensitivity and simple operation.

Background of Standard Formulation

With the increasing attention paid to healthy diet, cholesterol content has become one of the important indicators for measuring the quality of poultry eggs. The formulation of this standard aims to provide the industry with a unified detection method to ensure the consistency and accuracy of data.

Technical Principle Analysis

Steps	Description	Key Reagents
Saponification	The sample is treated with potassium hydroxide solution and methanol to hydrolyze cholesterol esters into free cholesterol.	Potassium hydroxide solution (600 g/L)
Extraction	Isolate cholesterol by ether extraction to remove impurities.	Ether (C₄H₁₀O)
Concentration	Evaporate to near dryness and make up to volume with anhydrous ethanol.	Rotary evaporator

Implementation suggestions

In actual operation, it is recommended that:

Select reagents and instruments strictly in accordance with standard requirements.
Calibrate equipment regularly to ensure measurement accuracy.
Establish a blank control group to reduce errors.

More samples: GB 50021-2001(2009) DL/T 368-2010 QC/T 942-2021

Sample only — not a preview of GB/T 37077-2018

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