GB/T 33117-2016 in English
VALIDDetection and identification of Helgardia herpotrichoides?
- Issued on:2016-10-13
- Implemented on:2017-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$243.00
《GB/T 33117-2016小麦基腐病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
1. Background and significance of the standard
GB/T33117-2016 "Identification Method for Quarantine of Wheat Basal Rot" is an important standard formulated by my country for wheat basal rot (Helgaria herpotrichioides), aiming to standardize the quarantine process and detection technology of the pathogen. This standard was proposed and coordinated by the National Technical Committee for Plant Quarantine Standardization, and the drafting units include Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Qingdao Inspection and Quarantine Technology Development Center, etc.
2. Basic information of wheat basal rot pathogen
| Classification information | Transmission route | Host range |
|---|---|---|
| Scientific name: Helgaria herpotrichioides (Fron) Crous & W. Gam | The diseased remains are mixed among seeds | Gramineae plants such as wheat and Secale |
| Synonym: Cercospora herpotrichioides Fron | - | Barley, cultivated two-row barley, etc. |
3. Analysis of quarantine identification methods
On-site quarantine: According to SN/T2122 and SN/T2589, focus on whether there are suspected lesions on the stems and leaf sheaths of the host plant.
Laboratory quarantine: Comprehensive judgment is made through symptom inspection, PCR initial screening test and hyphae morphology observation.
Case analysis: Isolation and culture of wheat base rot pathogen
Taking soil samples as an example, the selective PDA plate separation method was used to culture the pathogen. The results showed that at 15℃, the pathogen formed characteristic brown colonies, which were easy to distinguish from other common plant pathogens.
4. Comparison of standard frameworks
| Dimensions | GB/T33117-2016 | SN/T2589 | Comparative analysis |
|---|---|---|---|
| Detection method | PCR gel electrophoresis + culture trait observation | Pathogen morphological identification | GB/T33117 is more comprehensive, combined with molecular biology technology |
| Scope of application | Gramineae such as wheat and Secale | Various plant pathogenic fungi | More targeted, specifically for wheat basal rot |
5. Implementation Suggestions
Staff training: Strengthen technical training for quarantine personnel to ensure that they are proficient in PCR detection and separation and culture techniques.
Equipment update: Equipped with advanced molecular biology equipment (such as fully automatic DNA extraction instrument, gel imaging system) to improve detection efficiency.
International cooperation: Strengthen technical exchanges with major wheat exporting countries to promote mutual recognition and cooperation in quarantine standards.
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
GB/T 39916-2021 in English
Guidelines for the establishment of plant quarantine system in entry and exit container depot
2021-04-30 -
GB/T 44619-2024 in English
Detection and identification of Pomacea Perry
2024-09-29 -
GB/T 23617-2009 in English
General principles of survey on forestry quarantine pest
2009-04-27 -
GB/T 15791-2011 in English
Rules of monitoring and forecasting for the rice sheath blight (Rhizoctonia solani Kukn)
2011-09-29 -
GB/T 23416.1-2009 in English
Safety technical specification of pest control for vegetables—Part 1:General principles
2009-03-28 -
GB/T 27614-2011 in English
Guidelines for import and release of biological control agents and other beneficial organisms
2011-12-30 -
GB/T 19495.4-2018 in English
Detection of genetically modified organisms and derived products—Qualitative real-time polymerase chain reaction(PCR) methods
2018-09-17 -
GB 7411-2009 in English
Quarantine protocol for cotton seeds in producing areas
2009-04-27
