GB/T 35829-2018 in English

Professional interpretation of the determination methods of 4 kinds of naphthalene diols in cosmetics

1. Overview of the standard and scope of application

GB/T 35829-2018 specifies the high performance liquid chromatography (HPLC) determination method of 1,7-naphthalene diol, 2,3-naphthalene diol, 1,5-naphthalene diol and 2,7-naphthalene diol in cosmetics, which is applicable to the detection of non-wax-based creams, emulsions, gels, water-based and powder cosmetics.

2. Background of standard formulation and technical evolution

1,7-naphthalene diol and 2,3-naphthalene diol are prohibited substances in my country's "Technical Specifications for Safety of Cosmetics (2015 Edition)". Since they may exist as impurities in cosmetics, the formulation of this standard provides a scientific basis for the detection of these substances. With the rapid development of the cosmetics industry, efficient and accurate detection methods have become an industry demand.

3. Detection principle and steps

Different extraction methods are used according to the type of cosmetics: creams, lotions and water samples are directly extracted with 95% ethanol; powder samples are extracted with a mixture of 95% ethanol and 0.1% acetic acid solution. After centrifugation and filtration, the extract is measured by high performance liquid chromatography, and qualitative analysis is performed in combination with retention time and spectrum.

4. Experimental conditions and equipment

The experiment used a high performance liquid chromatograph (HPLC) equipped with a diode array detector. The reference working conditions included: C18 column, mobile phase gradient elution, column temperature 30℃, flow rate 1.0 mL/min, wavelength 230 nm.

5. Data analysis and quality control

The standard working curve was drawn by diluting the mixed stock solution, and the peak area of the sample solution was used for quantitative analysis. The result calculation formula is as follows:

6. Practical application and case analysis

In actual detection, if the response value of the substance to be measured in the sample solution exceeds the linear range of the standard curve, it needs to be appropriately diluted with 95% ethanol before measurement. Positive samples need to be confirmed by HPLC-MS/MS.

7. Implementation Suggestions