GB/T 36191-2018 in English
VALIDProtocol of diagnostic methods for red sea bream iridovirus disease(RSIVD)
- Issued on:2018-05-14
- Implemented on:2018-12-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$175.00
《GB/T 36191-2018真鲷虹彩病毒病诊断规程》由TC156（全国水产标准化技术委员会）归口，TC156SC11（全国水产标准化技术委员会水产养殖病害防治分会）执行，主管部门为农业农村部。
Introduction
1. Background and significance of the standard
GB/T 36191-2018 "Diagnostic Procedure for Red Sea Bream Iridescent Virus Disease" is one of the important standards in my country's aquatic products sector, mainly used to standardize the diagnostic method of Red Sea Bream Iridescent Virus Disease (RSIVD). The standard was proposed by the Ministry of Agriculture and Rural Affairs and is managed by the National Technical Committee for Aquatic Products Standardization, which has important guiding significance for the industry.
2. Comparison of standard frameworks
| Standard dimensions | Scope of application | Detection methods | Technical requirements |
|---|---|---|---|
| Clinical diagnosis | Aquatic animals | Histopathology detection + PCR verification | Combination of symptom observation and laboratory analysis |
| Virus isolation | MFF-1 cell line culture | CPE observation + IFAT test | Aseptic operation and positive control |
| PCR detection | Nucleic acid extraction and amplification | Primer design + Electrophoresis verification | 570 bp specific fragment amplification |
3. Implementation recommendations
3.1 Laboratory operation recommendations
Reagent preparation: Ensure that all reagents such as primers, PCR reaction system and cell culture medium meet the standard requirements, especially the source of RSIV reference strains must be certified by the relevant departments of the Ministry of Agriculture.
Instrument calibration: Fluorescence microscopes, inverted microscopes and PCR instruments must be calibrated regularly to ensure detection accuracy.
3.2 Disease monitoring recommendations
For susceptible fish such as flounder and red sea bream, it is recommended to establish a normalized monitoring mechanism. Sampling should be strictly carried out in accordance with GB/T18088 and SC/T 7103 to ensure the representativeness and standardization of samples.
3.3 International Trade Precautions
Before exporting aquatic products, RSIVD testing must be completed and a negative certificate must be provided. If the importing country has special requirements, additional verification can be performed by referring to the ISKNV testing method.
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