GB/T 37908-2019 in English

VALID

General analysis regulation of the label-free protein microarray based on imaging ellipsometry

Issued on:2019-10-18
Implemented on:2020-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 37908-2019
Document status:	VALID
Title in English:	General analysis regulation of the label-free protein microarray based on imaging ellipsometry
Title in Chinese:	基于光学椭偏成像的无标记蛋白质芯片分析方法通则
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-10-18
Implemented on:	2020-05-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	C30-Medical apparatus and devices in general
Professional Classification:	GB-National Standard
Related Keywords:	label-free protein chip analysis methods label-free protein chip analysis method label-free protein microarray general analysis regulation practical application cases label-free protein microarray
Related Topics:	chip about protein protein analysis Two methods of ellipsometer protein chip light sheet imaging microquantum marker protein Protein Analysis Methods Ellipsometry-based Protein chip detection protein chip technology protein chip imaging chip protein chip protein chip c1 Blank Finally the most protein analysis method? in vitro marker protein protein chip Protein chip detection material imaging marker protein spectrophotometric protein Protein Analysis Methods photometric protein spectrophotometric protein reverse protein chip Ellipse Imaging Technology light sheet imaging in vivo protein labeling marker protein Image Analyzer Method optical chip Optical eccentric Optical eccentric protein spectrometry imaging chip Optical eccentric protein flakes optical chip protein molecule protein chip technology protein + spectrophotometry protein photometry Protein chip detection c6 Optical eccentric 90 degrees Optical - Polarized protein a UV-labeled protein protein spectroscopy Optical eccentricity meter protein analysis ChIP protein suspension protein chip c6 Incandescent Imaging Analyzer protein analysis Protein Luminosity Optical rotation of protein protein photometry protein composition Amino acid analysis method in protein Europium-labeled protein Core white light technology Image Analyzer Method protein spectroscopy unmarked GBT37908 Fluorescent protein labeling technology NH3 protein tag imaging ellipsometer How to prepare protein standard curve Correction methods for proteomic data Proteomics research methods protein analysis methods In vivo imaging analysis methods The methods that can be used to isolate and purify proteins are shotgun proteomic approach How to make a protein standard song Protein pathway analysis methods Mass spectrometry-based proteome research methods Proteomics analysis methods Elementary school science protein detection method Principles of protein blotting experimental methods Is proteomics a molecular biology method? The difference between protein precipitation method and chromatography method Fluorescence analysis method for animal live imaging Protein standard curve Proteomics analysis methods Chip foreign matter component analysis method Common methods for protein iodine labeling Blank matrix sample analysis and evaluation method Regulatory documents on analytical method deviations Mass spectrometry data processing methods for proteomics

《GB/T 37908-2019基于光学椭偏成像的无标记蛋白质芯片分析方法通则》由TC421（全国生物芯片标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

National Standard of the People's Republic of China GB/T 37908—2019

General Rules for Label-free Protein Chip Analysis Methods Based on Optical Ellipsometry

Background and Significance of Standard Formulation

The label-free protein chip analysis method based on optical ellipsometric imaging technology is an important innovation in the biomedical field in recent years. This technology realizes the detection of molecular interactions without sample labeling through optical measurement with high spatial resolution, and has the advantages of rapidity, sensitivity and high throughput. The formulation of this standard provides unified technical specifications for the research and development, production and application of related instruments, and promotes the widespread application of biochip technology in basic research and clinical medicine.

Standard dimensions	Technical requirements	Detection methods
Instrument performance indicators	Microfluidic pump flow range: $1~\mu L/min \sim 50~\mu L/min$, chip reactor unit volume: no more than $500~\mu L$	Use standard sheets (thickness $1~nm \sim 20~nm$) to perform detection dynamic range test and correlation coefficient calculation.
Detection sensitivity	Minimum detection limit: no less than $10~ng/mL$, repeatability error: less than $3\%$ within the chip, less than $10\%$ between chips	Verified by dilution of anti-IgG solution and repeated measurement.
System compatibility	Supports multi-channel operation, compatible with different sample input and detection requirements.	Compare test accuracy with other recognized methods to ensure consistency of results.

Explanation of professional terms and practical application cases

Label-free protein microarray: No physical, chemical or biological labels are required, and detection is performed directly through the specific binding of probe molecules to target molecules. For example, in the screening of disease markers, this technology can detect multiple candidate proteins at the same time.

Surface mass density: The mass of biomolecules on the chip surface per unit area is the core measurement parameter of optical ellipsometry technology.

Standard Framework Comparison and Technology Evolution Analysis

This standard is based on GB/T 1.1-2009 and combines the latest research results in the field of biochips at home and abroad. Compared with traditional fluorescent labeling technology, optical ellipsometry has the following advantages:

Non-contact detection: Avoid sample interference and damage.
High sensitivity: Can detect $10~ng/mL$ level target molecules.
Fast multi-throughput: Supports simultaneous detection of multiple indicators and samples.

Implementation Suggestions

To ensure the effective implementation of the standard, it is recommended that:

Instrument manufacturers conduct R&D and production in strict accordance with technical requirements.
Laboratories need to calibrate and verify the performance of equipment before use.
Strengthen technical personnel training and familiarize themselves with the standard content and testing procedures.

More samples: NB/T 47013.2-2015 YD/T 3934-2021 GB 50021-2001(2009)

Sample only — not a preview of GB/T 37908-2019

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