GB/T 38569-2020 in English

VALID

Performance evaluation of industrial microorganism strain—Raman spectroscopy

Issued on:2020-03-31
Implemented on:2020-03-31
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $80.00
$78.00

Standard No:	GB/T 38569-2020
Document status:	VALID
Title in English:	Performance evaluation of industrial microorganism strain—Raman spectroscopy
Title in Chinese:	工业微生物菌株质量评价拉曼光谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-03-31
Implemented on:	2020-03-31
Professional Classification:	GB-National Standard
Related Keywords:	performance evaluation industrial microorganism strain raman spectroscopy introduction analysis quality evaluation evaluation cycle quantitative evaluation system
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《GB/T 38569-2020工业微生物菌株质量评价拉曼光谱法》由424-cnis（中国标准化研究院）归口，主管部门为国家市场监督管理总局。

Introduction

Analysis of the core content of the standard

This standard innovatively applies micro-Raman spectrometer to the quality evaluation of industrial microbial strains, and realizes the prediction of fermentation performance through single-cell spectral similarity calculation. Technical principle is based on:

Raman spectral fingerprint characteristics of cellular compounds
400-1850cm^-1 characteristic peak shift analysis
Cosine distance algorithm to quantify similarity

Comparison of key technical parameters

Indicators	Requirements of this standard	Conventional methods
Spectral resolution	≥5cm^-1	Usually 10-15cm^-1
Number of cells detected	≥20/sample	Population detection is the main method
Qualified threshold	R<0.05	No quantitative standard

Key points for implementation of the standard

Typical application scenarios

An amino acid production company adopted this standard to evaluate the mutant strain of Corynebacterium glutamicum:

Cultivated to the stable period (OD₆₀₀=12.5)
Collected 35 single-cell spectral data
Calculated R value was 0.032±0.004

After fermentation verification, the difference in acid production with the reference strain was ≤2.7%, verifying the reliability of the standard.

Technology Evolution Analysis

This standard breaks through the limitations of traditional culture methods:

Time efficiency: shortens the evaluation cycle from 72 hours to 8 hours
Sensitivity: can detect 0.1% difference in metabolite composition
Standardization: establishes a quantitative evaluation system for the first time

More samples: JC/T 2453-2018 JJG 21-2008 DL/T 5154-2012

Sample only — not a preview of GB/T 38569-2020

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