GB/T 39030-2020 in English

Analysis of the core content of the standard

This standard establishes a physiological activity evaluation system for three types of enzyme preparations: lipase, protease and carbohydrate hydrolase. It simulates the animal/human digestive environment through an in vitro digestion model and adopts the gastric-intestinal two-stage digestion method to determine the digestion promotion rate.

Comparison of key technical indicators

Implementation points

Analysis of standard evolution

This standard is the first to establish an evaluation system for the physiological activity of enzyme preparations rather than simply the enzyme activity. The innovations include:
1. Introducing an in vitro digestion model to simulate the real digestive environment
2. Defining the calculation formula for digestion promotion rate: P=(W₂-W₁)/W₀×100%
3. Distinguishing the evaluation conditions of the two types of feed/food simulants

Application suggestions

1. The experiment needs to be repeated ≥3 times, and the residue needs to be cleaned with acetone and dried at 105℃
2. The result should include: enzyme preparation name, manufacturer, concentration, three major nutrients content and digestion promotion rate
3. It is recommended to cooperate with the standard operation of gastric/intestinal simulation solution preparation in Appendix A/B