GB/T 42080.2-2022 in English

Specimen Collection

Specimen collection should select appropriate tissue type and size according to the purpose of the test. The collection information should include the donor's health status, medication history and surgical records.

Isopentane (C5H12) is used for rapid freezing to reduce the risk of protein degradation.

Fresh Tissue Transportation

Use a cooling box or vacuum packaging to maintain the ambient temperature at 2℃~8℃. Record the transportation time and the temperature change around the container.

Reference studies have shown that maintaining 0℃~4℃ conditions can significantly improve protein stability (see Appendix A for examples).

Specimen processing and freezing

Pathological evaluation should be performed immediately after specimen receipt, and suitable tissue areas should be selected for protein isolation.

Use liquid nitrogen or dry ice to quickly freeze sections to avoid the effects of warm ischemia and cold ischemia on protein spectra.

Protein separation and detection

Proteins should be separated using commercial kits or laboratory-developed procedures. Western blot or Bradford method is recommended for quantitative analysis.

The separated proteins should be tested as soon as possible. If long-term storage is required, it is recommended to be dispensed into cryogenic vials (≤-70℃).

Quality Control

Validate the entire workflow, including specimen collection, freezing, and protein separation steps. Document variables that cannot be fully controlled (e.g., cold ischemia time) and develop risk mitigation measures.

Staff Training

Ensure that operators are familiar with standardized procedures, especially those involving hazardous chemicals such as isopentane.