GB/T 44293-2024 in English

VALID

Image measurement method for numerical aperture of microscopic objective

Issued on:2024-08-23
Implemented on:2025-03-01
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $310.00
$301.00

Standard No:	GB/T 44293-2024
Document status:	VALID
Title in English:	Image measurement method for numerical aperture of microscopic objective
Title in Chinese:	显微物镜数值孔径图像式测量方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2024-08-23
Implemented on:	2025-03-01
ICS Classification:	31.260-Optoelectronics. Laser equipment
Chinese Classification:	L50-Optoelectronic device assembly
Professional Classification:	GB-National Standard
Related Keywords:	image measurement method numerical aperture image numerical aperture numerical aperture circle nominal numerical aperture
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《GB/T 44293-2024显微物镜数值孔径图像式测量方法》由TC487（全国光电测量标准化技术委员会）归口，主管部门为中国科学院。

Introduction

GB/T 44293—2024 Standard Overview

This standard specifies in detail the image measurement method for the numerical aperture of microscope objective lenses, which is applicable to infinity-corrected microscope objective lenses with a nominal numerical aperture greater than 1.00. High-precision measurement is achieved through innovative imaging technology combined with the principle of total reflection.

Measurement Principles and Key Technologies

Based on the principle of optical total reflection, the parallel light emitted by the measuring device passes through the objective lens to be measured and forms two circular patterns on the lower surface of the cover glass: the numerical aperture circle and the total reflection circle. These patterns are captured by an image sensor, and the diameter ratio is calculated using professional software to finally obtain the numerical aperture of the microscope objective lens.

Composition of the measurement device

Module name	Function description
Imaging illumination module	Provides a transmitted imaging light source for observing the cover glass pattern.
Focusing module	Precisely adjusts the distance between the objective lens and the cover glass to ensure clear imaging.
Measurement illumination module	Outputs a high-precision parallel beam to meet measurement requirements.
Detection module	Composed of a tube lens and an array image sensor, it is used to capture measurement images.

Measurement environment requirements

Temperature:23°C ±5°C
Relative humidity:20% ~70%
Air pressure:86 kPa ~106 kPa

Measurement steps

Install the objective lens and cover glass to be measured, and add immersion liquid.
Turn on the imaging light source and adjust the focus to a clear state.
Switch to the measurement light path and capture the numerical aperture image.
Process the image through the test software and calculate the numerical aperture.

Uncertainty analysis

According to the JJF1059.1-2012 standard, the measurement uncertainty consists of Class A (repeatability) and Class B (circle fitting accuracy). The calculation formula for the combined uncertainty is as follows:

$$ U_{\mathrm{NA}} = \overline{{\mathbf{NA}}} \sqrt{\left({\frac{U_{D_{\boldsymbol{\psi}}|}}{D_{\boldsymbol{\psi}}|}}\right)^2 + \left({\frac{U_{D_{\boldsymbol{\psi}}|}}{D_{\boldsymbol{\psi}}|}}\right)^2} $$

Wherein, $$ U_{\mathrm{A}} = t_p u_A $$ and $$ U_{\mathrm{B}} = g \frac{a}{k} $$ represent Type A and Type B expanded uncertainties, respectively.

Implementation recommendations

Precautions for practical application:

Ensure that the cover glass and immersion liquid meet standard requirements (such as refractive index, thickness).
Regularly calibrate the measurement device, especially the performance of the light source and image sensor.
It is recommended to maintain constant environmental conditions during the measurement to reduce external interference.
Operators need to be professionally trained and familiar with measurement principles and equipment maintenance.

More samples: GB 50021-2001(2009) DL/T 5154-2012 GB 18306-2015

Sample only — not a preview of GB/T 44293-2024

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