SN/T 0533-2016 in English

VALID

Method for the determination of ethoxyquin residue in fruits for export

Issued on:2016-12-12
Implemented on:2017-07-01
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $310.00
$301.00

Standard No:	SN/T 0533-2016
Document status:	VALID
Title in English:	Method for the determination of ethoxyquin residue in fruits for export
Title in Chinese:	出口水果中乙氧喹啉残留量检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2016-12-12
Implemented on:	2017-07-01
Superseding:	SN 0533-1996 Method for the determination of ethoxyquin residues in fruits for export
Professional Classification:	SN-Import&Export Inspection
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Introduction

Analysis of the Evolution of Standard Technology

Technical Indicators	SN 0287-1993	SN 0533-1996	SN/T 0533-2016
Detection Method	Liquid Chromatography-UV Method	Fluorescence Spectrophotometry	HPLC-FLD/LC-MS/MS
Lower Limit of Determination (mg/kg)	0.1	0.2	0.05
Applicable Matrices	Single Fruit	Single Fruit	Five Types of Fruit

Principle and Key Technical Points

Method 1: HPLC-FLD

A C18 column (250mm×4.6mm, 5μm) was used with an acetonitrile-water gradient elution. The fluorescence detector parameters were: excitation wavelength 360nm, emission wavelength 435nm. The retention time of ethoxyquin was approximately 10.8 minutes.

Method 2: LC-MS/MS

An electrospray ionization source (ESI+) was used, monitoring ion transitions were 218.1→174.2 (quantification ion) and 218.1→148.2, with collision energies of 30 V and 21 V, respectively. The column was shortened to 150 mm, and the flow rate was reduced to 0.4 mL/min.

Key Operating Specifications

Sample Pretreatment Differences:

Apple/Pear/Peach/Plum: 1% Vitamin C was added for antioxidant effect

Citrus: Direct homogenization

The extraction solvent was n-hexane. 4 mL of the supernatant was concentrated for HPLC-FLD, and 1 mL for LC-MS/MS.

Method Validation Data

Fruit Type Spike Concentration(mg/kg) Recovery Range(%) RSD(%)

Apple 0.05 90.0-108.0

1.0 79.4-97.5

6.0 92.7-101.5

Implementation Recommendations

LC-MS/MS is recommended for citrus samples to avoid matrix interference

When testing low-concentration samples, the HPLC-FLD method should pay attention to the fluorescence quenching effect

Standard solutions should be stored in the dark and should be prepared immediately before use

More samples: JJG 21-2008 GB 18306-2015 JC/T 2453-2018

Sample only — not a preview of SN/T 0533-2016

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