SN/T 4784-2017 in English

VALID

Qualitative detection of rJorovirus and Hepatitis A virus in food for export一Real一time RT-PCR

Issued on:2017-05-12
Implemented on:2017-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $210.00
$204.00

Standard No:	SN/T 4784-2017
Document status:	VALID
Title in English:	Qualitative detection of rJorovirus and Hepatitis A virus in food for export一Real一time RT-PCR
Title in Chinese:	出口食品中诺如病毒和甲肝病毒检测方法实时RT-PCR方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-05-12
Implemented on:	2017-12-01
Professional Classification:	SN-Import&Export Inspection
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Introduction

Technical Background of the Standard

This standard addresses the detection of norovirus and hepatitis A virus in food for export. It establishes a rapid detection method using real-time RT-PCR technology. Issued and implemented in 2017, it replaces traditional detection methods, achieving a sensitivity of 10¹ to 10³ copies/μL.

Core Detection Process

Amplification procedure: 55℃ reverse transcription for 60min → 95℃ pre-denaturation for 5min → 45 cycles (95℃ for 15s, 60℃ for 1min)

Interpretation criteria:

Positive judgment: Ct value ≤ 40 and amplification curve is S-shaped
Suspicious result: 40＜Ct≤45 requires repeated testing
PC recovery rate should be ≥1%

Implementation points

Cross-contamination prevention and control: Experimental partitions implement GB 19489 requires that the nucleic acid extraction and amplification areas be physically isolated.
Primer verification: The specificity of GI/GII primers needs to be verified to avoid nonspecific amplification.
Sensitivity verification: Use ATCC standards to establish a standard curve with R²＞0.98.

Comparison of technological evolution

Steps	Norovirus	Hepatitis A Virus	Quality Control
Sample Pretreatment	PEG/NaCl Precipitation	TGBE Buffer Extraction	Process Control Materials (PC)
RNA Extraction	Guanidine Lysis Method	Magnetic Bead Method	Exogenous RNA Control (ECRNA)
Primer Design	G1/GII Type-Specific Probe

Indicators	Traditional ELISA	Conventional RT-PCR	This standard method
Detection time	24-48h	6-8h	3-4h
Sensitivity	10⁴ virus particles	10² copies	10¹ copies
Type differentiation	Untypable	Needs electrophoresis confirmation	Multiple fluorescence differentiation

More samples: NB/T 47013.2-2015 JGJ 94-2008 JJG 21-2008

Sample only — not a preview of SN/T 4784-2017

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