GB/T 32946-2016 in English
VALIDDetermination of proline in honey - High performance liquid chromatography
- Issued on:2016-08-29
- Implemented on:2017-03-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 32946-2016蜂蜜中脯氨酸的测定 高效液相色谱法》由TC601（全国蜂产品标准化技术委员会）归口，主管部门为中华全国供销合作总社。
Introduction
GB/T 32946-2016 Interpretation of the Standard
This standard specifies the HPLC method for the determination of proline in honey, and is applicable to the determination of proline in honey.
Background of Standard Formulation
GB/T 32946-2016 "High Performance Liquid Chromatography Method for the Determination of Proline in Honey" was proposed by the All-China Federation of Supply and Marketing Cooperatives and is under the jurisdiction of the National Bee Products Standardization Working Group. The drafting units of the standard include the Bee Research Institute of the Chinese Academy of Agricultural Sciences, the Bee Products Quality Supervision, Inspection and Testing Center of the Ministry of Agriculture (Beijing) and the Chinese Beekeeping Society.
Technology Evolution Analysis
High Performance Liquid Chromatography (HPLC) is a highly sensitive and efficient analytical technique that is widely used in the field of food testing. This standard uses fluorescence detectors and external standard method for quantification, combined with pre-column derivatization technology, to achieve accurate quantification of proline in honey.
Comparison of standard frameworks
| Standard dimensions | GB/T 32946-2016 | International integration | Scope of application |
|---|---|---|---|
| Method principle | High performance liquid chromatography, fluorescence detection | In line with relevant ISO standards | Applicable to all types of honey samples |
| Reagent requirements | Analytical pure reagents, first-grade water | Consistent with international testing specifications | Laboratory use |
| Instruments and equipment | High performance liquid chromatograph (with fluorescence detector) | Meet the requirements of modern analytical technology | Standardized laboratory configuration |
Implementation suggestions
1. Sample preparation and storage:
- Samples need to be stored at 4°C to avoid deterioration.
- The sampling volume is recommended to be no less than 0.5kg to ensure experimental repeatability.
2. Extraction steps:
- Weigh 1g of sample, accurate to 0.001g.
- Add boric acid buffer to dissolve and then ultrasonically extract for 10 minutes.
- Filter and derivatize.
3. Derivatization and detection:
- Use NBD-F solution for pre-column derivatization, and the reaction time is 10 minutes.
- The excitation wavelength of the fluorescence detector is 470nm, and the emission wavelength is 530nm.
- The standard working curve should cover the range of 0.625mg/L to 20.00mg/L.
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