GB/T 37545-2019 in English
VALIDDetermination of 38 kinds of permitted colorants in cosmetics—High performance liquid chromatography
- Issued on:2019-06-04
- Implemented on:2020-01-01
- File Format:PDF
- Delivery:Via email within 5 business days
$301.00
《GB/T 37545-2019化妆品中38种准用着色剂的测定 高效液相色谱法》由TC257（全国香料香精化妆品标准化技术委员会）归口，TC257SC2（全国香料香精化妆品标准化技术委员会化妆品分会）执行，主管部门为中国轻工业联合会。
Introduction
Analysis of standard technical framework
| Technical elements | GB/T 37545-2019 | International general method |
|---|---|---|
| Detection range | 38 approved colorants (excluding lakes) | Usually 20-30 |
| Pretreatment method | Simultaneous extraction in tetrahydrofuran-methanol-water system | Mostly step extraction |
| Chromatographic conditions | ZORBAX SB-Aq column, 32min gradient elution | C18 column is the main one |
Core technology innovation
This standard innovatively uses the combination of high performance liquid chromatography-diode array detector and quadrupole mass spectrometer to break through the three major limitations of traditional detection methods:
- Breakthrough in solubility barrier: Synchronous extraction of water/oil-soluble colorants is achieved through the tetrahydrofuran-methanol-water ternary system
- Improved detection efficiency: 38 compounds can be analyzed in a single injection, which is 300% more efficient than traditional methods
- Enhanced confirmation capability: Establish an MRM mass spectrometry database to provide a basis for secondary mass spectrometry confirmation
Key operating points
Typical application cases
A lipstick sample was found to have excessive Acid Red 52 (actual value 620μg/g, limit value 500μg/g):
- Verified using Agilent 1260 HPLC system
- Mass spectrometry confirmation parameters: parent ion m/z 557.1→daughter ion 512.8 (collision energy 50eV)
- Finally determined to be an unqualified product
| Key steps | Technical parameters | Precautions |
|---|---|---|
| Sample preparation | 0.2g sample + 6mL tetrahydrofuran | Lipstick requires 80℃ water bath to assist dispersion |
| Nitrogen blow concentration | Concentrate to 0.5mL | Avoid overdrying |
| Chromatographic analysis | Injection volume 2μL | Test must be completed within 10h |
Standard implementation recommendations
Laboratory configuration plan
- Basic configuration: HPLC-DAD system (Agilent/ Waters recommended)
- Confirmation configuration: LC-MS/MS (AB Sciex 5500 or equivalent)
- Consumables requirements: PTFE filter membrane (0.45μm)
Main points for quality control
- Standard solution should be prepared and used immediately (shelf life ≤ 3 months)
- Spiked recovery should be performed for each batch of samples (required 90-110%)
- Regularly check the chromatographic column efficiency (theoretical plate number ≥ 5000)
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