GB/T 38579-2020 in English
VALIDDetermination of photosynthetic bacteria in biologic products
- Issued on:2020-03-31
- Implemented on:2020-03-31
- File Format:PDF
- Delivery:Via email within 1~3 business days
$272.00
《GB/T 38579-2020生物产品中光合细菌测定》由424-cnis（中国标准化研究院）归口，主管部门为国家市场监督管理总局。
Introduction
Analysis of the core content of the standard
| Detection object | Identification method | Culture conditions | Characteristic indicators |
|---|---|---|---|
| Rhodospirilla crimson | MNP labeling + carbon source utilization | 30℃ microaerobic | Spiral cells, ethanol positive |
| Rhodopseudomonas palustris | 10 MNP sites | 2000lx light | Citrate utilization positive |
Technological Breakthroughs and Innovations
This standard establishes for the first time a multiple nucleotide polymorphism (MNP) identification system, which achieves interspecies identification through 22 specific marker sites. Compared with traditional physiological and biochemical methods:
- The detection cycle is shortened from 7 days to 3 days
- The identification accuracy is increased to 99.7%
- The detection limit is up to 10 CFU/g
Key Operation Points
Specifications for Culture Medium Configuration
Photosynthetic bacteria isolation agar needs to be added with 5 mL of ascorbic acid supplement (Appendix A.2), and the temperature needs to be kept below 46°C when pouring the plate. The double-layer plate method can effectively inhibit the growth of miscellaneous bacteria.
Molecular identification quality control
MNP detection requirements:
1. DNA purity A260/280 ≥ 1.7
2. Sequencing coverage ≥ 700X
3. Detection of ≥ 3 marker sites can be considered positive
Implementation suggestions
- Anaerobic environment must be maintained during sampling, and it is recommended to use pre-reduced phosphate buffer
- The colony spread samples should be pretreated by the membrane filter method
- Establish a standard strain quality control library and regularly verify the sensitivity of the reagents
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