GB/T 39101-2020 in English

VALID

Determination of antimicrobial activity for polypeptides—Inhibition zone method

Issued on:2020-09-29
Implemented on:2021-04-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 39101-2020
Document status:	VALID
Title in English:	Determination of antimicrobial activity for polypeptides—Inhibition zone method
Title in Chinese:	多肽抗菌性测定抑菌圈法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-09-29
Implemented on:	2021-04-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Keywords:	polypeptides inhibition zone method introduction interpretation inhibition zone clear inhibition zone automated inhibition zone common problem solving blurred inhibition zone edge
Related Topics:	Novel Antimicrobial Peptides antibacterial antimicrobial peptide F1 Zone of inhibition Mycosporin Mycosporin Quantification of peptide concentration Peptide Sequencer peptide tagged peptide Peptide multiple charges Peptide mapping Peptide sequencing polypeptide Signal peptide prediction Peptide sequencing Antibacterial endogenous peptide pre-diffusion letter peptide Molecular Peptide Test Method lowry+ method peptide antibacterial Peptides Antibacterial detection Antibacterial 3 items Zone of inhibition standard Aldehyde peptide Antibacterial detection bacteria method hplc peptide How to measure the zone of inhibition Peptide assay dipeptide polarity predicted signal peptide Peptide mass spectrometry+ Golden inhibition zone Gold zone of inhibition Peptide Mass Spectrometry resistant bacteria dipeptide polarity Peptide Mass Spectrometry common linear peptide Antigenic peptide Antimicrobial peptide action Signal peptide prediction Determination of short peptides by hplc sex peptide Peptide writing chain peptide cyclic peptide Peptide polarity Peptide + mass spectrometry peptide array Multidimensional peptide Multidimensional peptide Zone of inhibition based on zone of inhibition method Zone of inhibition analysis animal polypeptide Zone of inhibition analysis Peptide assay lcms peptide chain Peptide jump Inhibition zone related Peptide series western peptide Mass Spectrometry Peptides superbug antimicrobial peptides peptide hplc Polypeptide animal Peptide Isolation Method Peptide + Source Quantification of short peptides Zone of Inhibition Test Method Zone of Inhibition Test Method Polypeptide liquid phase Antimicrobial Peptide Superbugs Peptide Technology Polypeptide Determination method of short peptide Peptide Actual Principle of zone of inhibition Determination of short peptides antimicrobial peptides peptidoglycan Zone of inhibition test Zhongshan peptide peptide properties Peptide short peptide 2a short peptide 2a zone of inhibition method lipopeptide antigenic peptide antigen peptide lcms Peptide Qualitative Detection Zone of inhibition test Broad-spectrum antibacterial detection fungal diversity Antigenic peptide+ Peptide analyzer How much primary antibody and secondary antibody to add Multiple Antigenic Peptides polypeptide chain peptide antigen Antibacterial Standard Cyclic peptide Peptide UV peptide sequencing Peptide fungal diversity peptide sequencing Peptide fast Cyclic lipopeptide Antimicrobial Peptide Superbugs superbug antimicrobial peptides Small peptide identification anti-peptide antibody Determination of peptides in serum Principle of zone of inhibition Quantification of Peptides by Fluorescence Determination method of peptide in serum Antibody peptide Antimicrobial Peptides Core polypeptide Small peptide assay method Liquid peptide The characteristics of the guide peptide Antimicrobial peptide action peptide bond peptide domain Peptide Bond Assay peptide sequence polarity of the dipeptide Peptide Mapping peptide chirality specific peptide Inhibition Zone Determination Method Peptides... mbp peptide Medical Antimicrobial Peptides l-type bacteria multiple inhibition Antibacterial peptide sterilization time Human peptide Clinical Antimicrobial Peptides Peptide Sequencer In vitro zone of inhibition test method How to measure fungi Research Peptides antimicrobial peptides in pesticides Peptide mass spectrometry more than 64 Determination method of short peptide concentration triple peptide Berry bacteria antibody Peptide Grass Peptide method Antibacterial zone diameter peptide antibacterial animal polypeptide antigenic peptide binding simple peptide Bacterial Cyclic peptide cleveland method peptide Lactobacillus peptide sex peptide Peptides by Mass Spectrometry Peptide Map Peptide Map porous peptide natural antimicrobial peptides peptinomycin polarity of the peptide chain peptide bond amphoteric anti-lymogenous peptide Antigenic peptide detection interface peptide antiviral peptide Triketone Peptides plasma antimicrobial peptides Antigenic peptide analysis GBT39101 GB/T 39101-2020 Antibacterial with silver peptide Peptide measurement method Folin phenol method for determination of peptide content Inhibit free radicals and antibacterial ppm antibacterial Measure OD600 to measure the antibacterial rate Antimicrobial peptide in vivo experiments LinTT1 polypeptide D-type polypeptide WB monoclonal and polyclonal antibodies Inhibition zone measurement Peptide screening Overlapping Inhibition zone positive control Antibacterial properties after charging Quality of antimicrobial peptides Characterization methods of peptides Peptide industry Peptide content determination method Peptide detection method Drug susceptibility test inhibition zone Method for determination of peptide content Peptide testing industry Determination of inhibition zone of doxycycline Antibacterial International Soil Antimicrobial Test Determination of inhibition zone diameter Peptide content determination method Polypeptide production method Peptide detection methods Measurement of inhibition zone antibiotic inhibition zone Inhibition zone tester calibration standard plate Determination of antibacterial efficacy Peptide content testing method Methods for sterility testing to eliminate bacteriostasis Peptide activity detection method Calibration of inhibition zone tester Peptide enrichment methods Peptide method development Antimicrobial peptide potency Europe Inhibition Zone Calibration Peptide content detection method Peptide modification methods Peptide testing methods Peptide purification methods Inhibition zone diameter Peptide content detection method Peptide optical rotation Methods to detect peptide content Antibacterial Ring International Antigenic peptide analysis method Peptide determination method Methods for detecting peptide concentration UV detection method of peptides Peptide quantitative detection method: fine Peptide purification methods Methods for purifying peptides Calibration method of inhibition zone measuring instrument Peptide identification methods Peptide concentration detection method Peptide biological activity assay method gbt39101-2020 Peptide mapping method specificity Oxytetracycline inhibition zone Phage antibacterial activity gb/t 39101-2020

《GB/T 39101-2020多肽抗菌性测定抑菌圈法》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Interpretation of the core content of the standard

This standard specifies a standardized method for determining the antibacterial activity of polypeptide substances using the Oxford cup diffusion method, and calculates the antibacterial potency (AU/mg) by measuring the diameter of the inhibition zone.

In-depth analysis of technical principles

Based on the diffusion characteristics of polypeptides in agar plates: the effective antibacterial components diffuse from the Oxford cup to the surrounding culture medium, forming a concentration gradient, and a clear inhibition zone is generated when the local concentration is higher than the minimum inhibitory concentration.

Test type	Indicator bacteria requirements	Culture conditions	Judgment criteria
Antibacterial performance	1-5×10⁸CFU/mL	36±1℃ normal culture	R²≥0.9900
Antifungal performance	1-5×10⁷CFU/mL	29±1℃ normal culture	R²≥0.9900

Key Operation Points

Sample Preparation Case

Take bacitracin detection as an example: accurately weigh 10.0 mg of sample, dissolve it in phosphate buffer (pH 7.2) and dilute it in a gradient to 0.0625-2.0 mg/mL, ensuring that the diameter of the inhibition zone is within the linear range of 8.00-25.00 mm.

Quality assurance measures

Use a vernier caliper (precision 0.02mm) for three-point measurement
Set up 3 parallel replicates for each concentration
Standard strain ATCC25923 is used as a quality control reference

Recommendations for the implementation of the standard

The laboratory should establish a standard strain library and regularly verify the temperature uniformity (±1℃) of the constant temperature incubator. It is recommended to use an automated inhibition zone measuring instrument to improve data reproducibility.

Common Problem Solving

Blurred inhibition zone edge: Check the culture medium thickness (20mL/90mm plate) and pre-diffusion time (4-10h)
Poor linearity: Optimize the concentration gradient range to ensure that the inhibition zone covers 8-25mm

More samples: QC/T 942-2021 GB 50021-2001(2009) NB/T 47013.2-2015

Sample only — not a preview of GB/T 39101-2020

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