GB/T 40256-2021 in English
VALIDCode of diagnosis for marteiliosis of oysters—Microscopic methods
- Issued on:2021-05-21
- Implemented on:2021-12-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 40256-2021牡蛎马尔太虫病诊断规程 显微镜检查组织法》由TC156（全国水产标准化技术委员会）归口，TC156SC11（全国水产标准化技术委员会水产养殖病害防治分会）执行，主管部门为农业农村部。
Introduction
Technical Background of the Standard
Martei disease is an important parasitic disease of bivalve molluscs and is listed by OIE as a reportable aquatic animal disease. This standard replaces part of SC/T7207.3-2007 and standardizes the microscope tissue method as an independent diagnostic method for the first time, solving the technical connection problem between on-site rapid detection and laboratory diagnosis.
Comparison of core detection methods
| Technical parameters | Tissue print detection method | Histopathological detection method |
|---|---|---|
| Detection cycle | 2-3 hours | 48-72 hours |
| Worm detection stage | Early stage (4-8μm) and cyst stage (20-40μm) | Worms at various developmental stages of the digestive tract |
| Staining characteristics | Giemsa staining: cytoplasmic blue/nuclear red | H&E staining: basophilic cytoplasm/eosinophilic nucleus |
| Diagnostic value | On-site screening | Basis for diagnosis |
Key technical points
1. Sample processing specifications
Differentiate sampling according to the growth stage of shellfish: Visceral mass is taken from juvenile shellfish, Stomach/intestine/digestive gland/adductor muscle needs to be separated from adult shellfish. DAFA fixative (ethanol-formalin-acetic acid system) must be prepared and used immediately, and the fixation time should not exceed 24 hours.
2. Microscopic diagnostic features
The development of refractory Maltese shows obvious stages:
• Larva stage: 4-8μm in diameter, mostly distributed in the gastric epithelium
• Cyst stage: 20-40μm in diameter, concentrated in the digestive gland
• Diagnostic structure: refringent bodies are the key identification feature
Implementation recommendations
- Seasonal prevention and control: Strengthen monitoring when the water temperature is ＞17℃, and increase the sampling frequency during the high temperature period in summer
- Result cross-validation: It is recommended that positive samples be reviewed in combination with the SC/T7207.3-2007 electron microscopy method
- Biosafety: Live shellfish from the epidemic area need to be isolated and observed to prevent the introduction of type O/M strains
Technology Evolution Analysis
This standard has major improvements over the 2007 version:
1. Added Mayer-Bennett Hematoxylin staining formula (Appendix A.2)
2. Clarified the thresholds for determining parasites at different developmental stages
3. Added molecular biological confirmation requirements for suspected cases in non-endemic areas
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
GB/T 28630.1-2012 in English
Diagnostic protocols for white spot disease—Part 1:Dot blot hybridization method with DNA probe
2012-07-31 -
GB/T 36190-2018/XG1-2020 in English
Protocol of diagnostic methods for hemorrhagic disease of grass carp,includes Amendment 1
2020-12-14 -
GB/T 37115-2018 in English
Protocol of diagnosis for infection with abalone herpersvirus
2018-12-28 -
GB/T 20443-2006 in English
Method for the determination of diethylstilbestrol in chicken tissue by high performance liquid chromatography with electrochemical detection
2006-08-28 -
GB/T 41185-2021 in English
Specification of preparation and quality control of reference materials for DNA detection of aquatic animals pathogen―Plasmid
2021-12-31 -
GB/T 37746-2019 in English
Triplex RT-PCR assay for detection of grass carp reoviruses
2019-06-04 -
GB/T 25878-2010 in English
Polymerase chain reaction(PCR) method for infectious hypodermal and haematopoietic necrosis virus(IHHNV)
2011-01-10 -
GB/T 42821-2023 in English
Diagnostic methods for bonamiosis of molluscs
2023-08-06
