GB/T 42956-2023 in English

1. Overview and Standardization Background

This standard GB/T 42956-2023 specifies the determination of tylosin, tyvalosin and tilmicosin in feed, using liquid chromatography-tandem mass spectrometry (LCMS). This method is suitable for the detection of veterinary antibiotic residues in compound feed, concentrated feed, concentrate supplements and additive premixes.

With the development of modern animal husbandry, antibiotics are increasingly used in feed, but their residues may pose a threat to human health and the ecological environment. Therefore, it is particularly important to establish a scientific and accurate determination method.

2. Comparison of standardization frameworks

3. Experimental reagents and materials

The reagents required for the experiment include:

• Acetonitrile: chromatographic grade, used for extraction and purification
• Formic acid: chromatographic grade, used for solution preparation
• Potassium dihydrogen phosphate solution: concentration 0.1 mol/L

Standards include tylosin, tyvalosin and tilmicosin. Attention should be paid to the conversion of their hydrochloride forms.

4. Experimental instruments and equipment

5. Suggestions for the implementation of the standard

To ensure the effective implementation of the standard, it is recommended that:

• The laboratory should be equipped with instruments and equipment that meet the requirements
• Operators need to undergo professional training
• Regularly calibrate instruments to ensure detection accuracy
• Establish a sound quality control system