SN/T 5145.6-2019 in English

Analysis of standard technical framework

Detailed explanation of the method principle

Using double-labeled PCR technology:

1. The 5' end of the upstream primer is labeled with FITC fluorescein
2. The 5' end of the downstream primer is labeled with biotin
3. The amplification product is detected by immunochromatography test strips:
   • Gold-labeled anti-FITC antibody captures the fluorescent labeled end
   • Fixed streptavidin captures the biotin labeled end

Key operating points

Technology evolution analysis

Main improvements of this standard compared with the 2010 version:

• Detection limit increased from 1% to 0.1%
• Introduce universal test strips to replace electrophoresis detection
• Add sequencing verification step (NCBI homology ≥97%)

Implementation suggestions

1. Before testing processed foods:
   • Salt washing (high-salt products)
   • Pigment adsorption treatment (fermented products)
2. Recommended test settings for each batch:
   • Positive control (beef powder standard)
   • Process control (spiking recovery experiment)