SY/T 7471-2020 in English

VALID

Detection method of near-surface indicator microorganisms for oil and gas exploration

Issued on:2020-10-23
Implemented on:2021-02-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	SY/T 7471-2020
Document status:	VALID
Title in English:	Detection method of near-surface indicator microorganisms for oil and gas exploration
Title in Chinese:	近地表油气指示微生物检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-10-23
Implemented on:	2021-02-01
Professional Classification:	SY-Oil & Gas
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Introduction

Interpretation of the core content of the standard

This standard systematically regulates the entire process of near-surface sample collection and microbial detection in oil and gas microbial exploration for the first time, focusing on establishing a standardized operating system for two key indicator bacteria: methane oxidizing bacteria and butane oxidizing bacteria.

Technical Parameters Comparison

Test Items	MOB Standard Parameters	BOB Standard Parameters
Cultivation Gas Concentration	10% Methane	10% Butane
Cultivation Temperature	30±1℃
Effective Counting Range	30-300 Colonies/Dish

Key Technical Innovations

1. Stratified sampling specifications: For the first time, the sampling depth gradient (30-100cm) of different geological environments (plain/desert/seabed) was clarified

2. culture medium components Precise ratio: MOB culture medium contains 7 inorganic salt components, and BOB culture medium is optimized to 5 core components

3. Establish a dry soil correction formula: Eliminate the influence of soil moisture content on the test results through formula (2) (3)

Implementation suggestions

1. Special attention should be paid to the sampling process:
- It is recommended to use refrigerated sampling boxes for transportation in desert areas
- Seabed samples must be pretreated within 4 hours

2. Key points of quality control:
- Blank control culture dishes must be carried out simultaneously
- Temperature parameters of high pressure sterilizers must be calibrated monthly

More samples: JC/T 2453-2018 JJG 21-2008 NB/T 47013.2-2015

Sample only — not a preview of SY/T 7471-2020

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