GB/T 13093-2023 in English
VALIDDetermination of bacterial count in feeds
- Issued on:2023-08-06
- Implemented on:2024-03-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$146.00
《GB/T 13093-2023饲料中细菌总数的测定》由TC76（全国饲料工业标准化技术委员会）归口，主管部门为国家标准委。
Introduction
1. Standard background and update description
GB/T 13093-2023 "Determination of total bacterial count in feed" is an important update of the national standard, replacing the previous GB/T13093-2006 version. This standard mainly optimizes the method for determining the total bacterial count in compound feed, concentrated feed, concentrate supplements and feed raw materials of animal and plant origin.
2. Comparison of standard frameworks
| Standard dimensions | 2006 version content | 2023 version update |
|---|---|---|
| Scope of application | Mainly applicable to compound feed and concentrated feed | Extended to compound feed, concentrated feed, concentrate supplements and animal and plant feed raw materials |
| Experimental principle | Based on the dilution plate count method | Optimized the culture medium preparation and colony counting method |
| Key reagents | Detailed formula not specified | Newly added requirements for the preparation of standardized reagents such as phosphate buffer solution |
3. Detailed explanation of experimental steps
Preparation of sample solution:Use aseptic operation to weigh 25g of sample, add it to 225mL of sterile phosphate buffer solution or physiological saline, and evenly process it with a slapping homogenizer or homogenizing bag to make a 1:10 sample solution.
Dilution steps:Use the 10-fold incremental dilution method, and replace the sterile pipette or pipette tip at each step. Select 2-3 appropriate dilutions based on the expected contamination situation, and perform a blank control.
Culture conditions:After pouring the culture medium, invert the plate and culture it in a 36±1℃ constant temperature incubator for 48±2 hours to form colonies.
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