GB/T 19495.9-2017 in English

VALID

Detection of genetically modified organisms and derived products―Liquid bead array detection for plant products

Issued on:-
Implemented on:2018-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $100.00
$97.00

Standard No:	GB/T 19495.9-2017
Document status:	VALID
Title in English:	Detection of genetically modified organisms and derived products―Liquid bead array detection for plant products
Title in Chinese:	转基因产品检测植物产品液相芯片检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Implemented on:	2018-07-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Keywords:	genetically modified products detection technology plant products fluorescence detection technology liquid phase chip detection methods liquid-phase chip detection method gene chip detection method
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《GB/T 19495.9-2017转基因产品检测植物产品液相芯片检测方法》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

GB/T 19495.9—2017 In-depth interpretation of liquid phase chip detection methods for genetically modified products

Background and significance of the standard

The rapid development of genetically modified technology has promoted changes in the global agriculture and food industry, and has also brought about important needs for the detection of genetically modified ingredients. As an important part of China's national standards, GB/T 19495.9—2017 aims to provide scientific and standardized methods for the qualitative detection of genetically modified ingredients in plants and their products.

This standard focuses on liquid phase chip technology, which is an efficient, sensitive and high-throughput detection method. Through fluorescently encoded microspheres and flow cytometry, multiple target DNA sequences can be quickly identified and quantified. This method has significant advantages in the field of genetically modified detection, especially in large-scale sample screening.

Comparative Analysis of Standard Frameworks

Standard Number	Scope of Application	Detection Method	Main Features
GB/T 19495.1	General Requirements and Definitions for Detection of Genetically Modified Products	Basic Framework and Terminology Specifications	Laying the Foundation for the Standard System
GB/T 19495.6	Gene Chip Detection Method for Detection of Genetically Modified Products	Detection Technology Based on Solid Phase Chip	High-throughput, Multi-target Detection
GB/T 19495.9	Liquid-phase chip detection method for plant products	Fluorescence detection technology based on liquid-phase microspheres	High sensitivity and rapid screening

Working principle of liquid-phase chip technology

The core of liquid-phase chip technology lies in the preparation and detection of fluorescent-encoded microspheres. Each microsphere has a specific probe on its surface for capturing the target DNA sequence. The spectroscopic fingerprint of the microsphere and the bound reporter molecule are detected by red and green lasers, respectively, to achieve qualitative and quantitative analysis.

The following are the key steps of liquid-phase chip detection:

DNA extraction and preparation: According to the standard requirements, use the kit to extract the DNA in the target sample and ensure its purity and concentration.
PCR amplification: Amplify the target gene fragment, including the endogenous control gene (18s rRNA) and the exogenous screening element (CaMV35S promoter and NOS terminator) through polymerase chain reaction (PCR).
Hybridization reaction: Combine the PCR product with the specific probe in the liquid phase chip to form a stable hybrid chain.
Fluorescence detection: Detect the fluorescence signal of the microspheres by flow cytometry, and analyze the median fluorescence intensity (MFI) to determine the presence or absence of the target gene.

Implementation recommendations and precautions

Case study: Detection of genetically modified corn

In actual application, a testing agency used this standard to conduct liquid phase chip testing on a batch of imported genetically modified corn samples. The positive results of endogenous genes (18s rRNA) and the positive signals of exogenous screening elements (CaMV35S) confirmed that the samples contained genetically modified components. The detection sensitivity reached 0.01%, which meets the international standards.

This case shows that:

Strictly following standard operating procedures is the key to ensuring detection accuracy.
The setting of positive controls and negative controls helps to exclude false positive and false negative results.
Liquid phase chip technology has significant advantages in high-throughput detection and is suitable for large-scale sample screening.

More samples: NB/T 47013.2-2015 QC/T 942-2021 DL/T 368-2010

Sample only — not a preview of GB/T 19495.9-2017

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