SC/T 3053-2019 in English

VALID

Determination of astaxanthin in fish and fishery products by high performance liquid chromatography method

Issued on:2019-08-01
Implemented on:2019-11-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $130.00
$127.00

Standard No:	SC/T 3053-2019
Document status:	VALID
Title in English:	Determination of astaxanthin in fish and fishery products by high performance liquid chromatography method
Title in Chinese:	水产品及其制品中虾青素含量的测定高效液相色谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-08-01
Implemented on:	2019-11-01
Professional Classification:	SC-Aquaculture
Related Keywords:	high performance liquid chromatography high performance liquid chromatography method introduction analysis fishery products standard technical elements requirements new products
Related Topics:	Use of HPLC liquid product Principle of Liquid Chromatography Chromatographic element content sc test liquid color Use of High Performance Liquid Chromatography Chromatographic samples Principle of Liquid Chromatography high performance liquid chromatography gc content HPLC English sc element HPLC samples Determination of Water Soluble Vitamins by High Performance Liquid Chromatography Aquatic products liquid product Astaxanthin detection HPLC columns and their HPLC content Determination method of liquid phase content sc element Liquid phase content determination method Liquid phase content determination method Chromatographic samples Liquid + Products Astaxanthin Determination of Naphthalene Content by RP-HPLC HPLC liquid color liquid + color LC product water High performance liquid phase content determination method Astaxanthin detection HPLC a Water Content Chromatography Determination of Astaxanthin in Haematococcus by Liquid Chromatography sc solution Su Feng Pigment Content Spectrum sc solution element sc Determination method of high performance liquid chromatography Azadirachtin HPLC Zhong Su Astaxanthin 5 Efficient water content detection method Chromatogram sample Liquid Chromatography Measuring Range Astaxanthin' Liquid phase content measurement deviation for the same sample HPLC Does the product content in HPLC have an impact on the HPLC column? Astaxanthin coloring effect Test product purity using HPLC Qualitative methods commonly used in chromatographic analysis and their characteristics HPLC Astaxanthin quality Thallium content in aquatic products

Introduction

Analysis of the technical framework of the standard

Technical elements	Requirements of this standard	Internationally commonly used methods	Innovation points
Extraction solvent	Acetone/dichloromethane-methanol mixture	Pure acetone	Improve the extraction efficiency of fat-soluble components
Saponification conditions	React at 4-5℃ overnight	2 hours at room temperature	Reduce isomer conversion
Detection limit	2.5-10mg/kg	5-20mg/kg	Sensitivity increased by 2-4 times

Key technical points

High performance liquid chromatography uses a three-gradient elution system (methanol-tert-butyl methyl ether-phosphoric acid solution), which can effectively separate the three astaxanthin isomers of 13-cis, all-trans and 9-cis. In practical applications, special attention should be paid to the centrifuge speed of Antarctic krill samples, which must reach more than 8,000r/min to ensure complete extraction.

Recommendations for the implementation of the standard

Laboratories should establish a standard verification system to ensure that the purity of all-trans-astaxanthin standards is ≥95%
For high-content samples (＞100mg/kg), it is recommended to use dichloromethane-methanol solution for step-by-step extraction
Regularly calibrate the temperature stability of the nitrogen blowdown instrument to prevent thermal degradation of the target

Technology evolution analysis

This standard has major improvements over the 2009 version: ①Add a PSA filler purification step to eliminate phospholipid interference; ②Use a low-temperature saponification process (4-5℃) to reduce the isomer conversion rate by 40%; ③Expand the scope of application to new products such as krill oil.

More samples: DL/T 5154-2012 NB/T 47013.2-2015 GB 18306-2015

Sample only — not a preview of SC/T 3053-2019