SN/T 5115-2019 in English

VALID

Determination of carbadox in edible animal and feeds for import and export- LC-MS/ MS metho

Issued on:2019-09-03
Implemented on:2020-03-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $150.00
$146.00

Standard No:	SN/T 5115-2019
Document status:	VALID
Title in English:	Determination of carbadox in edible animal and feeds for import and export- LC-MS/ MS metho
Title in Chinese:	进出口食用动物、饲料中卡巴氧测定液相色谱-质谱/质谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-09-03
Implemented on:	2020-03-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	traditional methods detection limit carbadox edible animal export- lc-ms/ ms mrm mode high antibody cross-reactivity rate key standard technology
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Introduction

Interpretation of standard technology

Technical elements	Characteristics of this method	Comparison with traditional methods
Detection limit	Carbadox 5.0μg/kg, MQCA 1.0μg/kg	ELISA method 10-50μg/kg
Pretreatment time	About 2 hours	4-6 hours
Specificity	Double confirmation in MRM mode	High antibody cross-reactivity rate

Key technological innovations

The Oasis MAX solid phase extraction column combined with the Tris buffer system (pH 9.6) was used for purification, which significantly improved the removal rate of fat-soluble impurities. In the optimization of mass spectrometry parameters, carbadox selected the parent ion m/z 263.2→231.2, and MQCA selected m/z 175.0→102.1 as the quantitative ion pair.

Key points for implementation

The sample needs to be enzymatically hydrolyzed with proteinase K at 45℃ for 2 hours
0.1% formic acid-acetonitrile (9:1) is used as the extraction solvent
The chromatographic column is C18 (100mm×2.1mm, 2.7μm)

Standard Evolution Analysis

Compared with GB/T 20746-2006, this standard adds:

Simultaneous detection of metabolite MQCA
Improved matrix-matched calibration curve method
Expanded to feed matrix (the original standard is limited to animal tissue)

More samples: GB 50021-2001(2009) YD/T 3934-2021 JGJ 94-2008

Sample only — not a preview of SN/T 5115-2019

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