SN/T 5442-2022 in English
VALIDDetermination of prothioconazole and its metabolite residues in foodstuffs of plant origin for export - LC-MS/MS method
- Issued on:2022-03-14
- Implemented on:2022-10-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$243.00
Introduction
Interpretation of the core content of the standard
This standard establishes an analytical method for the determination of thioconazole and its metabolites in plant-derived foods by liquid chromatography-tandem mass spectrometry. The main technical features include:
| Technical indicators | Parameter requirements | Method advantages |
|---|---|---|
| Limit of quantification (LOQ) | 0.005 mg/kg | Meet the most stringent MRL requirements |
| Recovery range | 60.2%-101.4% | Conforms to GB 2763 verification specifications |
| Chromatographic analysis time | 11 minutes/sample | High throughput detection |
Method principle and technical evolution
Compared with the traditional GC-MS method, the innovations of this standard are:
- Adopting electrospray positive and negative switching mode to simultaneously detect the parent compound (342.2→100.0) and metabolites (312.0→70.1)
- Optimizing the QuEChERS pretreatment scheme and adding a n-hexane deoiling step for oil crops
- Introducing ascorbic acid solution as an antioxidant to effectively protect thermally unstable compounds
Key operating points
Typical application case: wheat sample detection
In actual testing, it was found that the extraction efficiency of thioconazole in wheat matrix was affected by the following factors:
- Ultrasonic time needs to be strictly controlled at 10±1 minutes
- Centrifugation temperature ≤4℃ can reduce the degradation of the target
- The use of PSA+C18 composite purifier can effectively remove pigment interference
Implementation suggestions
Pay special attention when conducting laboratories:
| Risk links | Control measures |
|---|---|
| Stability of standard | Store at -20℃ away from light and verify purity before use |
| Matrix effect | Matrix matching standard curve must be used |
| Instrument sensitivity attenuation | QC sample monitoring every 20 pins |
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