WS/T 421-2024 in English

VALID

Standards for antifungal susceptibility testing of yeasts-Broth dilution method

Issued on:2024-04-02
Implemented on:2024-09-01
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $490.00
$476.00

Standard No:	WS/T 421-2024
Document status:	VALID
Title in English:	Standards for antifungal susceptibility testing of yeasts-Broth dilution method
Title in Chinese:	抗酵母样真菌药物敏感性试验标准肉汤稀释法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2024-04-02
Implemented on:	2024-09-01
Superseding:	WS/T 421-2013 Antifungal susceptibility testing of yeasts-Broth dilution method
ICS Classification:	11.020-Medical Sciences and health care facilities in general
Chinese Classification:	C50-Sanitation in general
Professional Classification:	WS-Hygiene
Related Keywords:	antifungal susceptibility testing ecv/ecoff antifungal drugs antifungal drugs rpmi-1640 drug susceptibility treatment yeasts-broth dilution method introduction analysis
Related Topics:	fungus dilution sensitivity sensitive How to dilute the primary antibody primary antibody dilution Primary Antibody Dilution Ratio How to dilute the primary antibody Dilution of standard samples drug in english How to dilute primary and secondary antibodies Primary Antibody Secondary Antibody Dilution Dilution Standard substance diluter Primary and Secondary Antibody Dilutions How to dilute the primary antibody How long to dilute the primary antibody primary antibody dilution Primary Antibody Dilution Secondary Antibody Dilution Anti-dilution secondary antibody dilution Dilution of primary and secondary antibodies fungal drugs Sample Dilution Method Dilution of primary antibody How to Dilute Primary and Secondary Antibodies Secondary antibody dilution according to primary antibody sample dilution new antibacterial drugs Dilution of primary antibody and secondary antibody Dilute the primary antibody Primary Antibody Diluter How to dilute the primary antibody Primary antibody diluted in milliliters Primary Antibody Dilution Dilution of primary and secondary antibodies Standard substance dilution Nickel release standard Dilution of Standards flesh-eating bacteria infection How much is the primary antibody diluted? release fermentation drug stakeout broth How to Dilute Primary and Secondary Antibodies Yeast-like bacteria Secondary antibody diluted according to primary antibody? yeast dilution Drug bacteria Primary Antibody Secondary Antibody Dilution fungal diversity Anti+Secondary Antibody+Dilution drug sensitive sample How to dilute the standard substance fungal drugs Dilution drug sensitivity How to dilute primary and secondary antibodies drug sensitivity Standard substance diluter drug sensitivity How to Dilute a pH Standard fungal diversity Standard substance dilution Yeast-like fungi drug stakeout true resistance Primary Antibody Secondary Antibody Dilution standard dilution method Yeast-like fungi in vitro diagnostics How to gradually dilute the standard substance Dilute primary and secondary antibodies Sample dilution ratio After dilution of the standard substance illegal yeast common drug susceptibility test How to dilute the primary and secondary antibodies Time fungal drugs standard dilution assay laboratory yeast How many times are the primary and secondary antibodies diluted? primary antibody dilution Four-channel dilution sampling method Drug sensitivity test accuracy Drug sensitivity ＜=2 Abbreviation for broth dilution method for drug resistance testing Antibiotic susceptibility assessment standard nccl The dilution drug susceptibility test is much higher than Antimicrobial susceptibility testing Drug susceptibility test inhibition zone Standard dilution water Antimicrobial drug susceptibility testing Drug sensitivity testing Drug susceptibility test sensitivity determination Antimicrobial susceptibility testing performed Neutralizing antibody dilution method Drug susceptibility test judgment standard table Yeast competent preparation method Antibody drug release assay method Drug sensitivity test standard table Standard sample dilution factor Drug susceptibility standardization Standard curve dilution step by step dilution Drug sensitivity test methods Drug sensitivity testing performed Standard requirements for diluted ammonia ws/t832-2024 How to dilute standard gas Reference table for drug sensitivity test criteria t/ws0007-2024 ws/t 839-2024

本标准规定了用经典的宏量肉汤稀释方法（Broth Macrodilution method）检测抗酵母样真菌的最低抑菌浓度（Minimal Inhibitory Concentration, MIC）的参考方法，并推荐了与本参考方法结果具有良好一致性的微量肉汤稀释方法（Broth Microdilution method）。
本标准适用于引起感染的酵母样真菌（包括念珠菌属某些种Candida spp.和隐球菌属某些种
Cryptococcus spp.）的药物敏感性试验，并推荐了某些相关抗真菌药物的肉汤稀释试验的最低抑菌浓度（MIC）质控参考范围、折点、结果解释性分类和流行病学界值。
本标准不适用于双相真菌如皮炎芽生菌Blastomyces dermatitidis、荚膜组织胞浆菌Histoplasma capsulatum及丝状真菌的检测。

Introduction

Analysis of the core content of the standard

Technical update	2013 edition	2024 edition
Definition of terms	8 terms	4 new terms such as ECV/ECOFF
Antifungal drugs	12 types	6 new types such as Rezafungin

Key operating points

Preparation specifications for antifungal drugs

RPMI-1640 broth culture medium must be prepared strictly according to Appendix A:

Water-insoluble drugs should be dissolved in DMSO
The storage solution concentration must be ≥100 times the detection range
Store at -70℃ for no more than 6 months

Result interpretation criteria

Drug category	Endpoint judgment criteria	Scoring rules
Amphotericin B	100% inhibition	0 points (completely clear)
Pyrrole/Flucytosine	50% inhibition	2 points (turbidity significantly decreased)

Implementation recommendations

Principles of drug susceptibility treatment for Candida auris

For drugs with provisional breakpoints (such as fluconazole MIC ≥ 32μg/ml):

It is recommended to combine ERG11 gene testing
Echinocandins are preferred for treatment
The strain needs to be preserved for review

More samples: QC/T 942-2021 GB 18306-2015 YD/T 3934-2021

Sample only — not a preview of WS/T 421-2024

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

WS/T 491-2024 in English

Guidelines for non-specific syphilis antibody testing
2024-05-09
WS/T 477-2015 in English

Quantitative D-dimer assay
2015-11-06