GA/T 1620-2019 in English

Analysis of Standard Technical Framework

Core operation points

Quality Control and Result Evaluation

The standard requires that parallel sample testing must be carried out, and the relative difference (RD) ≤20% is valid data. Qualitative judgment must meet the following requirements:

• Retention time deviation ±1%
• Detection of ≥3 characteristic ions
• Ion abundance ratio complies with the allowable deviation in Table 1

Implementation suggestions

Method optimization direction

For liver samples with complex matrices, it is recommended to:

1. Increase the amount of phosphate buffer (pH10) to 8mL
2. Extend the ultrasonic time to 20min
3. Use matrix-matched standard curve for calibration

Standard evolution trend

Main improvements of the current standard compared with the earlier method:

• Introduction of solid phase extraction technology to improve recovery rate
• Clear criteria for determining characteristic ion abundance ratio
• Unify column temperature programs for GC and GC-MS