GB/T 21323-2007 in English

《GB/T 21323-2007动物组织中氨基糖苷类药物残留量的测定 高效液相色谱-质谱/质谱法》由424-caiq（中国检验检疫科学研究院）归口，主管部门为国家市场监督管理总局。
本标准规定了动物组织中壮观霉素、潮霉素B、双氢链霉素、链霉素、丁胺卡那霉素、卡那霉素、安普霉素、妥布霉素、庆大霉素和新霉素10种氨基糖苷类药物残留量的高效液相色谱�泊�联质谱测定和确证方法。
本标准适用于动物内脏、肌肉和水产品中10种氨基糖苷类药物残留量的测定。

1 Scope

This standard specifies a method used to determine and confirm the residues of 10 aminoglycosides in animal tissues, such as spectinomycin, hygromycin B, dihydrostreptomycin, streptomycin, amikacin, kanamycin, apramycin, tobramycin, gentamicin and neomycin - High performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS).

This standard is applicable to the determination of residues of 10 aminoglycosides in animal innards, muscles and aquatic products.

2 Normative references

The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.

GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-1992, neq ISO 3696: 1987)

3 Principle

Aminoglycosides residues in specimen are first extracted with phosphate buffer and then purified with a C18 solid phase extraction column, and after concentration, by using the heptafluorobutyric acid as the ion-pairing reagent, they are determined and quantified with HPLC-MS/MS method and external standard method respectively.

4 Reagents and materials

Unless otherwise specified, analytically-pure reagents and Grade-I water defined in GB/T 6682 shall be adopted.

4.1 Methanol: HPLC grade.

Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Instruments
6 Preparation and preservation of specimens
7 Determination procedures
8 Limit of quantitation (LOQ)
9 Recovery rate and precision
Annex A (Informative) Reference mass spectrometry conditions for MS/MS determination
Annex B (Informative) Reconstructed ion chromatogram of quantitative ion pairs of standard samples