GB/T 26635-2011 in English

《GB/T 26635-2011动植物油脂 生育酚及生育三烯酚含量测定 高效液相色谱法》由TC270（全国粮油标准化技术委员会）归口，TC270SC2（全国粮油标准化技术委员会油料及油脂分会）执行，主管部门为国家粮食和物资储备局。

1 Scope

This standard specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).

For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.

Note: a suitable method involving a cold saponification procedure is described in Annex B for information only.

2 Normative references

The following normative documents contain provisions which, through reference in this standard, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections) to, or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. For undated references, the latest edition applies.

GB/T 15687 GB/T 15687 Animal and vegetable fats and oils - preparation of test sample (GB/T 15687-2008, ISO 661:2003, IDT)

3 Terms and definitions

For the purpose of this standard, the following terms and definitions apply.

3.1
tocol content
mass fraction of the individual tocols, determined using the method specified in this standard

Note: the content is expressed in milligrams per kilogram as a whole number.

4 Principle

Foreword II
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Precision
12 Test report
Annex A (Informative) Examples of chromatograms
Annex B (Informative) Saponification
Annex C (Informative) Results of interlaboratory tests
Bibliography