GB/T 33682-2017 in English

SUPERSEDED

General microganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry

Issued on:2017-05-12
Implemented on:2017-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $70.00
$68.00

Standard No:	GB/T 33682-2017
Document status:	SUPERSEDED
Superseded by:	GB/T 33682-2025 General microorganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
Superseded on:	2025-09-01
Title in English:	General microganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
Title in Chinese:	基质辅助激光解析电离飞行时间质谱鉴别微生物方法通则
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-05-12
Implemented on:	2017-12-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Keywords:	matrix-assisted laser desorption/ionization time general microganism identification method flight mass spectrometry introduction gb/t time-of-flight mass spectrometry mass spectrometry data
Related Topics:	Biomass Spectrometry Too many peaks in flight matrix laser mass spectrometry Laser Assisted Mass Spectrometry Portable Time-of-Flight Mass Spectrometry Mass Spectrometry Identification GB/T 33682-2017 liquid phase time-of-flight mass spectrometry GB/T+33682-2017 GB33682-2017 MS ionization voltage GB/T33682-2017 Microbial Flight Mass Spectrometry laser mass spectrometry time-of-flight mass spectrometry time-of-flight surface mass spectrometry time-of-flight mass spectrometry laser time of flight gb t33682 2017 Laser time-of-flight non-time-of-flight mass spectrometry field ionization Mass Spectrometry Ionization Species Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Secondary mass spectrometry analysis General principles of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of microorganisms time-of-flight mass spectrometry for microorganisms gbt 33682 2017 gb+t+33682+2017 GB/T 33682 Peptide MS analysis gb+t33682+2017 Microbial Mass Spectrometry Peptide Mass Spectrometry Analysis time mass spectrometry quadrupole time-of-flight mass spectrometry Microbial Mass Spectrometry mass spec biological Ion Trap - Time of Flight ms mass spectrometer time-of-flight matrix time-of-flight mass spectrometry Auxiliary matrix mass spectrometry Laser Desorption Ionization Time-of-Flight Mass Spectrometry quadrupole time-of-flight mass spectrometry Microbial time-of-flight mass spectrometry Quadrupole Linear Ion Trap Time-of-Flight Mass Spectrometry Mass Spec Microbiology gb33682 ion trap time of flight time-of-flight ion trap ion trap time-of-flight mass spectrometry Mass spec microplate Auxiliary mass spectrometry laser desorbed matrix Mass spectrometry secondary analysis field analysis mass spectrometry time-of-flight mass spectrometry matrix Mass Spectrometry Microbial Methods mass spec biological How long is the mass spectrometer ionic hydrazine time of flight Ionic hydrazine and time 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In-flight mass spectrometry analysis Ion trap/time of flight mass spectrometer flight mass spectrometer laser solution Various ionization methods for mass spectrometry field ionization mass spectrometry time-of-flight mass spectrometry for microorganisms Laser Assisted Analysis Analysis of MS/MS microbial time Mass Spectrometry Ionization Method For mass spectrometry time laser desorption mass spectrometry mass peak time matrix-assisted laser desorption ionization time-of-flight mass spectrometry matrix laser analysis analysis time mass spectrometry Microbial Flight Mass Spectrometry UV time-of-flight mass spectrometry field analysis ionization Mass Spec Microbiology Microdisk mass spectrometry laser ionization Foreign matrix-assisted laser desorption ionization time-of-flight mass spectrometry Pressure during mass spectrometry flight mass spectrometry microbiology time-of-flight mass analyzer matrix-assisted laser mass spectrometry time-of-flight mass spectrometry biological mass 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solution Laser Assisted Matrix Desorption Time-of-Flight Mass Spectrometry analytical ionization mass spectrometry Mass resolution ion trap time-of-flight multistage mass spectrometry Matrix-Assisted Laser Resolution Time-of-Flight Matrix Assisted Laser of Flight Mass Spectrometry Desorption Ionization Time matrix-assisted laser desorption ionization mass spectrometry Matrix Assisted Laser Desorption Time-of-Flight Mass Spectrometry Linear ion trap mass spectrometry and time-of-flight mass spectrometry Matrix Assisted Mass Spectrometry time-of-flight mass spectrometry ion trap Linear Ion Trap Time-of-Flight Mass Spectrometry Analysis of mass spectra is often based on matrix-assisted laser desorption mass spectrometry time-of-flight analyzer laser time-of-flight mass spectrometry laser analysis laser time mass spectrometry matrix-assisted ionization desorption time-of-flight mass spectrometry Mass spectrometer Analysis of mass spectrum matrix-assisted ion trap mass spectrometer time of flight Peptide + MS Ionization field analysis mass spectrometry ion trap mass spectrometry time-of-flight mass spectrometry time-of-flight mass spectrometry matrix matrix laser analysis time-of-flight mass spectrometry Microbial mass spectrometry+ Ionization Methods for Mass Spectrometry ionic hydrazine time of flight ion trap mass spectrometer time of flight Mass Spec + Micro General rules for matrix-assisted identification of microorganisms Mass Spectrometry Ionization Methods matrix-assisted time-of-flight mass spectrometry Miniature Time-of-Flight Mass Spectrometer time-of-flight mass spectrometry domestic matrix desorption laser time-of-flight mass spectrometry matrix-assisted Matrix Assisted Laser Desorption Ionization-of-Flight Mass Spectrometry laser assisted ionization thermal ionization mass spectrometry matrix-assisted laser desorption ionization mass spectrometry time of flight, ion trap time-of-flight mass spectrometry and ms molecular mass spectrometry How long is the substance Quadrupole Mass Spectrometry Time-of-Flight Mass Spectrometry Ion Trap Mass Spectrometer time-of-flight mass spectrometry ion What substance is spectroscopically analytical ionization matrix-assisted laser desorption/ionization mass spectrometry Types of flight mass spectrometry matrix Matrix-Assisted Laser Desorption Ionization-based Time-of-Flight Mass Spectrometry matrix-assisted laser desorption mass spectrometry spatially separated mass spectrometry In-flight mass spectrometry analysis time-of-flight mass spectrometry matrix-assisted laser field analysis ionization Time-of-Flight Mass Spectrometry and Ion Trap Mass Spectrometry laser analysis time of flight time-of-flight mass spectrometry quadrupole mass spectrometer time-of-flight mass spectrometry Mass Spectrometry and Time-of-Flight Mass Spectrometry Analysis of time-of-flight mass spectrometry time-of-flight mass spectrometry for proteins matrix-assisted laser desorption ionization Mass Spectrometry for Substance 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matrix laser desorption mass spectrometry quadrupole mass spectrometer time-of-flight mass spectrometry Biomass Spectrometry,,, Powering the Microbiome gbt 33682-2017 Perform mass spec identification Substance analysis and identification method Mass Spectrometry - Quadrupole Time-of-Flight time-of-flight and ionic hydrazine flight mass spectrometry and ion mass spectrometry matrix-assisted laser desorption ionization time mass spectrometry According to biological seven mass spectrometry flight mass spectrometry microbiology Matrix-assisted laser desorption ionization-time-of-flight/time-of-flight mass spectrometry Mass spectrometry base peak Time-of-Flight Mass Spectrometry and Dual Mass Spectrometry Matrix Assisted Laser Desorption Ionization-Time-of-Flight-Mass Spectrometry Matrix mass spectrometry derivatization time-of-flight mass spectrometry sort of Ion Hydrazine Time-of-Flight Mass Spectrometry time-of-flight mass spectrometry Matrix Assisted Laser Desorption-Time-of-Flight Mass Spectrometry flight mass spectrometry ion small time-of-flight mass spectrometer flight mass spectrometry matrix matrix desorption laser ionization time time-of-flight mass spectrometry time matrix-assisted laser desorption ionization mass spectrometry Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry mass spectrometry ionization Matrix Assisted Laser Desorption Ionization for Mass Spectrometry time-of-flight mass peak Assisted Laser Analytical Ionization microbial time of flight Substrates for time-of-flight mass spectrometry matrix-assisted laser desorption-time matrix time-of-flight matrix laser mass spectrometry quadrupole mass spectrometer ion trap mass spectrometer time-of-flight mass spectrometry ion time-of-flight laser analytical ionization laser-assisted matrix mass spectrometry How to analyze matrix time-of-flight mass spectrometry Time-of-Flight and Laser Assist matrix-assisted laser desorption mass spectrometry quadrupole time-of-flight mass 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laser desorption ionization time-of-flight mass spectrometer price Soft ionization method of mass spectrometry Mass spectrometry ionization method Mass spectrometry negative ion mode analysis Mass spectrometry third edition pdf Antu microbial mass spectrometer Matrix-assisted laser desorption ionization source equipment composition Principles of matrix-assisted laser desorption time-of-flight mass spectrometry The difference between time-of-flight mass spectrometry and triple quadrupole Methods for analyzing the structure of ion channels How to separate substances Comparison of microbial identification methods Open direct ionization mass spectrometry analysis method Microbial mass spectrometry Spectral identification methods for drugs are specified as ( Mass spectrometry soft ionization method Identification methods drug analysis time spectrum method Bioanalytical Methodology Matrix Effects Common methods for identifying drugs using spectroscopy Chromatographic method for identification of impurities in drugs Microbial identification methods Netdisk Biomass Spectrometry Technology and Methods Raster decryption identification method How to identify strong electrolytes Microbial mass spectrometry charges Time of flight mass spectrometry calibration Electrolyte industry Microbial lysis method Electrolyte industry Time of Flight Mass Spectrometry Industry Process-based mass spectrometry analysis method Microbial mass spectrometry industry Rhizosphere microbial isolation methods Mass spectrometric identification quality Analysis methods of liquid mass spectrometry Methods of identifying and classifying microorganisms Methods for detecting and identifying substances containing chlorine radicals Plant hormone identification method Microbial mass spectrometry quality Methods for identifying the types of microorganisms in culture media Microbial mass spectrometry industry What are the methods to identify microorganisms? Physical property methods that can be used for electrolytes

《GB/T 33682-2017基质辅助激光解析电离飞行时间质谱鉴别微生物方法通则》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

GB/T 33682—2017 Standard Overview

This standard specifies in detail the application methods of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in microbial identification, and is suitable for various laboratories to quickly identify microorganisms in samples.

Analysis of Standard Formulation Background and Technological Evolution

Dimensions	Traditional methods	MALDI-TOF MS technology
Identification speed	Slow, dependent on culture and biochemical reactions	Fast and direct, no culture required
Sensitivity	Low, large amount of samples required	High sensitivity, trace amounts can be detected
Cost efficiency	More consumables, long cycle	Fewer consumables, easy operation

Formation and application of microbial protein fingerprint

Microbial protein fingerprinting is a characteristic protein distribution formed based on the unique genetic characteristics of different species. Through MALDI-TOF MS technology, proteins can be directly extracted from colonies on the culture medium and mass spectrometry data can be generated.

Application case analysis

For example, in food testing, this standard method can be used to quickly identify Staphylococcus aureus (according to GB4789.10—2016) or other pathogens, significantly improving detection efficiency and accuracy.

Implementation recommendations

Laboratories should be equipped with MALDI-TOF MS equipment and establish a microbial fingerprint database.
Operators must undergo professional training to ensure the correct use of biosafety cabinets (GB19489).
Regularly calibrate instruments and update the standard strain library to ensure the accuracy of identification results.

More samples: JC/T 2453-2018 JJG 21-2008 NB/T 47013.2-2015

Sample only — not a preview of GB/T 33682-2017

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