GB/T 34795-2017 in English

VALID

Determination of the activity of transglutaminase

Issued on:2017-11-01
Implemented on:2018-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $70.00
$68.00

Standard No:	GB/T 34795-2017
Document status:	VALID
Title in English:	Determination of the activity of transglutaminase
Title in Chinese:	谷氨酰胺转胺酶活性检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-11-01
Implemented on:	2018-05-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	G66-Biochemical reagent and clinical analysis reagent
Professional Classification:	GB-National Standard
Related Keywords:	glutamine transaminase activity unit glutamine transaminase activity detection method background enzyme activity transglutaminase introduction national standard detection principle transglutaminase
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《GB/T 34795-2017谷氨酰胺转胺酶活性检测方法》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

National Standard of the People's Republic of China GB/T 34795—2017 Glutamine Transaminase Activity Detection Method

Background of Standard Formulation and Analysis of Technical Evolution

Glutamine transaminase is an important biochemical reagent widely used in food processing, pharmaceutical manufacturing and other fields. In order to ensure its activity and consistency in industrial products, the national standard GB/T 34795—2017 came into being. This standard was proposed and managed by the National Technical Committee for Biochemical Testing Standardization. The main drafting units include Shenzhen Institute of Metrology and Quality Inspection, etc.

Definition of glutamine transaminase activity unit

According to the definition in standard 3.1, the glutamine transaminase activity unit is the amount of enzyme required to catalyze the production of 1.0 μmol of isohydroxy acid salt per minute at 37°C and pH 6.0. This definition provides a clear quantitative basis for the detection method.

Detection Principle

Transglutaminase catalyzes N-benzyloxycarbonyl-L-glutamylglycine (CBZ-Gln-Gly) and hydroxylamine to generate L-glutamic acid-Y-monoisohydroxy acid. After the product is colored with ferric chloride, it shows a characteristic absorption peak at a wavelength of 525nm, so the enzyme activity can be calculated by the external standard method.

Instrument and Reagent Specifications

Instrument	Technical Requirements
pH Meter	0.01 Level
Electronic Balance	Sensitivity 0.0001g
Spectrophotometer	With 525nm wavelength detection capability

Implementation Suggestions

In actual operation, it is recommended that laboratories configure instruments and reagents in strict accordance with standard requirements and ensure accurate control of temperature and pH value. At the same time, calibrate the equipment regularly to ensure the accuracy of the test results.

More samples: QC/T 942-2021 JC/T 2453-2018 DL/T 368-2010

Sample only — not a preview of GB/T 34795-2017