LS/T 6139-2020 in English

VALID

Inspection of grain and oils—Rapid screen determination for organophophate and carbamate pesticide residues in grains

Issued on:2020-01-21
Implemented on:2020-07-21
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $140.00
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Standard No:	LS/T 6139-2020
Document status:	VALID
Title in English:	Inspection of grain and oils—Rapid screen determination for organophophate and carbamate pesticide residues in grains
Title in Chinese:	粮油检验粮食及其制品中有机磷类和氨基甲酸酯类农药残留的快速定性检测
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-01-21
Implemented on:	2020-07-21
ICS Classification:	67.040-food products in general
Chinese Classification:	X11-Grain processing and products
Professional Classification:	LS-Cereal
Related Keywords:	pesticide residues oils rapid screen determination grain circulation link rapid screening system dichlorvos residues
Related Topics:	Organic Food Testing Rapid detection of organic mercury Qualitative detection Carbamate detection Residue testing formic acid Organic Food Inspection Organic food testing basis organic food Checking Food Quality Drug Rapid Testing Carbamate Rapid Test Carbamate Pesticides Organophosphorus pesticides and carbamates Organophosphorus pesticide detection in water Organophosphorus and carbamate pesticides Carbamate Pesticides Carbamates Organophosphorus and carbamate pesticides Organic food testing Organophosphorus and carbamate pesticides methyl organophosphorus carbamic acid Rapid detection of pesticide residues Animal Organophosphate Rapid Detection Other amino acid detection Food Pesticide Testing Organic Food and Testing Grain Inspection Pesticides Principle of rapid detection ls/t 3245 Food organic testing and inorganic testing Pesticide Residue Detection Rapid detection of organophosphorus and carbamate pesticide residues in vegetables Potato food products Drug Rapid Testing Basis Product detection of 4 kinds of amino acids What are the food testing Pesticide six six rapid inspection Organophosphate phosphorus detection How to detect organic food organic food Rapid detection of organophosphorus and carbamate pesticide residues in vegetables Detection of inorganic phosphorus and organic phosphorus in water Urethane in food Potato food products Organic detection of food pesticide residues Drug rapid test box Organophosphate rapid test Organophosphorus and carbamate pesticide residues in vegetables LS/T 3211 National occupation of carbamate pesticides China Grain Testing Organophosphates OPEs and POPS Organophosphorus and carbamate pesticide residue detection Basic principles of enzyme inhibition method for determination of organophosphorus and carbamate pesticides Organophosphorus detection principle Organophosphate detection of biological samples Organophosphate residues National standards for organophosphate testing Grain inspection and calibration The latest testing standards for carbamate pesticides Organophosphoric acid and carbamate residues Detection methods for amino acids in pharmaceuticals Rapid detection method for aged grains Method for determination of carbamate in food Methods, principles and limitations of rapid detection of pesticide residues. Carbamate pesticide detection method What are the methods for rapid detection of pesticides? Methods for detection of carbamate pesticides in food Carbamate pesticide residue detection Pesticide residue inhibition rate Optical spectrometry for detection of organophosphorus pesticides What are the detection methods for amino acids? What are the methods for quick detection of pesticide residues? Food testing amino column Detection method for formic acid residues Phosphate ester testing national standard Organophosphate detection Other amino acid testing methods Detection of phosphorus in petroleum

本标准规定了粮食及其制品有机磷类和氨基甲酸酯类农药残留的快速定性检测的原理、试剂和材料、仪器和设备、样品制备和结果分析等内容。
本标准适用于粮食及其制品有机磷类和氨基甲酸酯类农药残留的快速定性检测。
本标准的方法检出限见附录A。

Introduction

Core Value and Application Scenarios of the Standard

This standard aims at the risk control needs of pesticide residues in the grain circulation link, and establishes a rapid screening system based on fluorescence detector. Compared with the traditional chromatographic method, the detection time is shortened from 4-6 hours to 30 minutes, which is particularly suitable for on-site rapid screening in the grain storage and processing links.

In-depth Analysis of Technical Principles

The detection is based on the enzyme inhibition method: organophosphorus/carbamate pesticides inhibit the activity of insect esterases, resulting in a decrease in the hydrolysis rate of fluorescent substrates.

Technical parameters	Traditional method	This standard method
Detection principle	Chromatographic separation	Enzyme inhibition reaction
Sensitivity	0.01-0.1μg/kg	0.1-75μg/kg
Sample cost	200-300 yuan	30-50 yuan

Key operating points

Case study: When a grain depot tested dichlorvos residues in rice, it was found that the following operational differences had an impact on the results:

When the incubation temperature was 35℃±1℃, the inhibition rate deviation was ≤5%
Adding the fluorescent substrate for more than 30 seconds would cause the signal to attenuate by 15-20%
When the sample size did not reach the 20-mesh standard sieve, the extraction efficiency decreased by 30%

Implementation suggestions and precautions

1. Matrix difference compensation: Different grain varieties should refer to Appendix C to select the corresponding limit enzyme inhibition rate. For example, the dichlorvos limit in brown rice is 45.6%, while that in corn is 50.6%

2. False positive processing: For suspected positive samples (inhibition rate exceeds the standard), GC-MS/MS should be used for confirmation in combination with the limit requirements of GB2763

3. Equipment calibration: Fluorescence detector needs to be verified monthly with standard products to ensure the accuracy of the 460-550nm excitation wavelength and 590nm emission wavelength

More samples: GB 50021-2001(2009) GB 18306-2015 JJG 21-2008

Sample only — not a preview of LS/T 6139-2020

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